Learning outcomes of the course unit
Fundamental preparation aimed to the choise of the analytical metods and basic knowledges of instrumental techniques.
Fundamental preparation in General, Inorganic and Organic Chemistry.
Course contents summary
Introduction to instrumental analytical methods
Classification of the instrumental analytical techniques.
Criteria for the choice of the analytical method with respect to the analytical problem.
Main parameters for the quality assurance of the analytical methods: linearity, accuracy, detection limit, quantification limit.
Potentiometry. Theoretical bases and instrumentation. Specific electrodes. Membrane electrodes. Potentiometric determination of pH.
Voltammetry. Analytical applications. Use of voltammetry as detection technique for chromatography.
Mechanisms of energy exchange between radiation and substances. Relationships between molecular energy and wavelength of the radiations
Uv-visible absorption and fluorescence spectroscopy
Absorption and fluorescence mechanisms. Instrumentation for absorption and fluorescence spectroscopy. Relationships between electronic structure and absorption wavelength. Concentration intensity relationships. Quantitative analysis.
Relationships between vibration modes and absorption bands. FT-IR instrumentation. Application to the identification of unknown sostances .
Atomic absorption and emission spectroscopy.
Features of atomic spectra. Instrumentation for atomic absorption and emission spectroscopy. Applications to determination of metals at trace level. Sample treatment procedures for metal determination in biological samples.
Mass spectrometry (MS)
Theoretical bases of the technique. Instrumentation: ionization sources and mass analyzers. Applications to analysis of organic substances.
General features of the chromatographic techniques. Gas chromatography (GC) and Liquid chromatography (HPLC). Classification of substances on the basis of their polarity.
Separation mechanisms. Stationary phases. Instrumentation; detectors. Coupling GC-MS. Application to the analysis of volatile compounds. Sampling procedures of volatile substances.
Separation mechanisms. Mobile and stationary phases. Adsorption, partition and size exclusion mechanisms. Instrumentation for HPLC; colums and detectors. Ion exchange chromatography; colums and mobile phases, electrochemical detectors. Applications to the analysis of non volatile substances and of inorganic anions.
Theoretical bases. Planar electroforesis. Capillary electroforesis Instrumentation; detection systems. Applications to the analysis of low- and high-molecular weight substances.
D.C. Harris, Chimica Analitica Quantitativa, Zanichelli, 2004.
D.A. Skoog, J.J. Leary, Chimica Analitica Strumentale, SES, 1995.
D.A. Skoog, D.M. West, F. J. Holler, S.R. Crouch, Fondamenti di Chimica Analitica, EdiSES, 2004.
K.A. Rubinson, J.F. Rubinson, Chimica Analitica Strumentale, Zanichelli, 2002.
Lectures, exercises, laboratory practical experiments
Valutation trough written and oral examinations