Learning objectives
<br />AIMS<br />Principles and methods for the laboratory diagnosis of infectious diseases <br />Aetiology, outline of pathogenesis, clinical samples, laboratory diagnostic procedures, interpretation of the results of microbiological and virological investigations to diagnose the infectious diseases. <br />The determination of susceptibility/resistance of the pathogenic agents to chemotherapeutics
Prerequisites
All the examinations expected to the term of the 4th year
Course unit content
<br />CONTENTS<br />Principles and methods for the laboratory diagnosis of infectious diseases:<br /> <br />- Demonstration of the pathogenic agent and its constituents in the pathological material<br />- Demonstration of a recent specific immune response<br />- The medical prescription of microbiological and virological investigations<br />- Collection, transport and preservation of the clinical samples for microbiological and virological investigations.<br /> <br />Aetiology, outline of pathogenesis, clinical samples, laboratory diagnostic procedures, interpretation of the results of microbiological and virological investigations regarding the diagnosis of: <br />- Infections of the respiratory apparatus<br />- Infections of the intestinal apparatus and related glands<br />- Systemic infections<br />- Infections of the cardiovascular apparatus<br />- Infections of the central nervous system<br />- Infections of the genitourinary apparatus and sexually-transmitted infections<br />- Infections of the skin and cutaneous adnexa<br />- Infections of the muscular-skeletal apparatus<br />- Infections of the eye and the ear<br />- prenatal, connatal and perinatal infections.<br /> <br />The nosocomial infections<br /> <br />The determination of susceptibility/resistance of the pathogenic agents to chemotherapeutics:<br />- Methods <br />- Interpretation of the results<br />- The dosage of chemotherapeutics in tissues and biological fluids.<br /> <br />Professionalizing training: Legend: 0: The student has seen the execution, 1: The student has executed at least one time, 2: The student is able to execute autonomously.<br /> CLINICAL MICROBIOLOGY<br /> Medical prescription of microbiological and virological investigations 2<br /> <br />Interpretation of the report of the microscopic examination for bacteria (Gram staining) 2<br /> <br />Interpretation of the report of the microscopic examination for bacteria (Ziehl-Nielsen staining) 2<br /> Interpretation of the report of the cultural examination for bacteria 2<br /> Interpretation of the report of the antibimicrobial susceptibility tests for bacteria 2<br /> Interpretation of the report for the serological diagnosis of syphilis 2<br /> Interpretation of the report for the diagnosis of mycobacterial infection 2<br />Interpretation of the report for the detection of bacetrial nucleic acids 2<br />Interpretation of the report of the cultural examination for 2<br />Interpretation of the report of the antibiogram for fungi 2<br />Interpretation of the report for the detection of anti-Toxoplasma gondii antibodies 2<br />Interpretation of the report for the detection of intestinal parasites 2<br />Interpretation of the report for the detection of intestinal parasites 2<br />Interpretation of the report for the detection of Leishmania 2<br />Execution of the thin peripheral blood smear for 0<br />Interpretation of the report for the detection of nucleic acids of protozoa 2<br /> Agarose gel Electrophoresis of nucleic acids of protozoa 0<br />Interpretation of the report for the detection of antigens of protozoa 2<br /> Interpretation of the report for the laboratory diagnosis of malaria 2<br /> Detection of arthropods in human samples (macroscopic examination) 1<br />Detection of elmynths (Cestodes, Nematodes) by macroscopic examination 1<br />Interpretation of the report for the diagnosis of viral hepatitis 2<br />Interpretation of the report for the detection of antigens of hepatitis viruses 2<br />Interpretation of the report for the detection of nucleic acids of hepatitis viruses 2<br />Interpretation of the report for the detection of anti-hepatitis viruses antibodies 2<br />Agarose gel Electrophoresis of nucleic acids of viruses 0<br />Interpretation of the report for the detection of viruses by electron microscopy 2<br />Interpretation of the report for the detection of anti-viruses antibodies 2<br />Interpretation of the report of the culture for viruses 2<br />Interpretation of the report for the detection of viral antigens 2<br />Interpretation of the report for the detection of antigens of HIV 2<br />Interpretation of the report for the detection of nucleic acids of HIV 2<br />Interpretation of the report for the detection of anti-HIV antibodies 2<br />
Full programme
- - -
Bibliography
<br />SUGGESTED REFERENCES<br /> <br />MICROBIOLOGIA CLINICA<br />ANNA M. M0LINA ROMANZI <br />Microbiologia Clinica<br />Gli aspetti microbiologici delle malattie infettive<br />UTET Periodici<br /> <br />
Teaching methods
<br />Oral examination<br />The practical laboratory activity includes the professionalizing training.
Assessment methods and criteria
- - -
Other information
- - -