Learning outcomes of the course unit
To show knowledge and understanding of the majormetabolic pathways, the molecular mechanisms of regulation, the generalprinciples of metabolic energy homeostasis and cell plasticity. To be able toresolve problems concerning the metabolic use of major food constituent in tissues.To be able to integrate carbohydrate, lipid and protein metabolism in a healthyindividual from a global and holistic point of view. To be able to resolveproblems concerning the endocrine regulation of the metabolic functions of theorganism, the metabolic coordination and the biochemical correlations betweenorgans and tissues under normal conditions and following hormonestimulation.
It is mandatory to pass the following exams 1)Chemistry and Propaedeutic Biochemistry and 2) Histology before being allowedto take the exam of Biochemistry.
Course contents summary
Bioenergetics and oxidative metabolism. Thetricarboxylic acid cycle. Electron transport chain. Oxidative phosphorylation.Glycolysis: the glycolytic pathway and regulation. Gluconeogenesis.Glycogenolysis and glycogenesis. Pentose phosphate pathway. Oxidation of fattyacids and aminoacids; metabolism of ketone bodies.
Glycogen metabolism: synthesis, breakdown andregulation. Metabolism of cholesterol and plasma lipoproteins. Lipolysis andlipogenesis. Metabolism of ammonia. Transport of nitrogen to liver and kidney.Synthesis and degradation of amino acids. Iron and heme metabolism. Purine andpyrimidine nucleotide metabolism. Endocrine metabolism: amines, peptidehormones, steroid hormones. Mechanism of hormone action. Hormonal control ofmetabolism.
Principles of nutrition. Digestion and absorption ofbasic nutritional constituents. Biochemistry of nutrients. Macro- andmicronutrients. Recommended levels of assumption of major nutrients (LARN orRDA): general principles and epidemiological aspects. General formulation ofdiets. Assessment of nutritional status in clinical practice. Principal typesof dietary regimes used in medicine. Food as vector of nutrients,anti-nutrients and not nutrients.
Baynes JW, Dominiczak MH: Biochimica per le discipline biomediche, secondaedizione, Casa Editrice Ambrosiana, Milano, 2006.
Bhagavan NV: Medical Biochemistry, fourth edition,Harcourt/Academic Press, Burlington, 2002.
Caldarera CM: Biochimica Sistematica Umana, seconda edizione, CLUEB, Bologna,2003.
Devlin TM: Textbook of Biochemistry with clinical correlations, fifth edition,Wiley-Liss, New York, 2002.
Garrett RH,Grisham CM: Principi di Biochimica, con messa a fuoco su quella umana, Piccin,Padova, 2004.
Mathews CK, van Holde KE, Ahern KG: Biochimica, terza edizione, Casa EditriceAmbrosiana, Milano, 2004.
Murray RK, Granner DK, Mayes PA, Rodwell VW; Harper Biochimica, 25a edizione,McGraw-Hill Libri Italia srl, Milano, 2000.
Nelson DL, Cox MM: I principi di Biochimica di Lehninger, terza edizione,Zanichelli, Bologna, 2002.
Siliprandi N, Tettamanti G: Biochimica Medica: strutturale, metabolica efunzionale, Piccin. Padova, 2005.
Voet D, Voet JG: Biochemistry, 3rd edition, John Wiley& Sons, USA, 2004.
Teachingactivity: frontal lessons, interactive activity for small groups, seminars,learning by solving problems
Kindof exam: written + oral test