ANALYTICAL CHEMISTRY II
Learning outcomes of the course unit
to provide theoretical knowledge regarding analytical techniques usually applied to food control and analyses
prerequisites are not requested
Course contents summary
Development and validation of instrumental analytical methods.; liquid membrane ion selective electrodes. Definition of validation requirements for analytical methods. specificity, analyte response linearity, accuracy, and precision. Lower limit of detection and quatitation. Quantitation procedures, Method of internal and external standard,. Use of reference standards. Matrix effects throughout the application of the methods. Sample preparation. Solid-phase extraction (SPE).. solid-phase micro extraction (SPME). Purge and trap desorption. Supercritical fluid extraction (SFE). Potentiometric sensors, ion selective electrodes, liquid membrane electrodes. Near-infrared spectroscopy (NIR). Chromatography, optimisation of the experimental conditions in GC and HPLC. Applications in food analyses. Size exclusion chromatography, calibration of the separation systems. Chiral recognization by HPLC separation methods.
Capillary electrophoresis (CE), Background Theory and Principles of CE. Electroosmotic flow (EOF), factors controlling EOF and its modulation. Electrocinetic micellar capillary chromatography (MECC) and capillary electrocromatography (CEC); theory and application, separation of ionic and no-ionic analytes.
Mass spectrometry, theory, principles and instrumentation. The ionization process, common ionization thechniques: electron impact (EI), chemical ionization (CI), electrospray ionization (ES), atmospheric pressure chemical ionization (APCI), thermospray and particle beam interfaces. Mass analyzers and detection mode. Mass spectrometer coupled with GC, HPLC and CZE. Nuclear magnetic resonance (NMR). Concepts and fundamentals, chemical shift. Instrumentation and applications in food analysis.
Skook, west, holler, Crouch, 'Fondamenti di Chimica Analitica', edizione 2004, Edises Napoli
R. Keller, j.-m. Mermet, M. Otto, H.m. Widmer, 'chimica analitica' edizione 2003, Edises, Napoli
The final oral exam focuses on all of the topics included in the course syllabus. The student must demonstrate that he/she has understood the fundamental concepts of each topic and that he/she is able to apply them.