PHYSICAL METHODS IN BIOINORGANIC CHEMISTRY
Learning outcomes of the course unit
To provide the students with theoretical and applicative notions on multidimensional NMR, with attention on paramagnetic, inorganic and bioinorganic systems.
Course contents summary
Monodimensional NMR spectroscopy. FT-NMR. Relaxation rates and their determination. Correlation time. Multidimensional NMR. COSY, TOCSY, HETCOR E HMQC spectra. Nuclear Overhauser Effect. NOESY spectra. Elements on analysis of parameters obtained by multidimensional NMR. Elements of n-dimensional experiments. NMR spectra of paramagnetic compounds. Elements on analysis of parameters obtained by NMR spectra of paramagnetic compounds of bioinorganic interest. DOSY and PGSE spectra. Determination and uses of translational diffusion coefficient in solution. Stability constants determination for host-guest systems by NMR.
Recommended readings
J. Keeler, Understanding NMR spectroscopy, John Wiley and Sons, 2005
I. Bertini, C. Luchinat, NMR of paramagnetic substances, Elsevier, 1996
