Learning outcomes of the course unit
Advanced concepts in optical spectroscopy and (multi)photonics
Course contents summary
Excitation energy transfer
- Förster and Dexter mechanisms
- Applications of FRET (Fluorescence Resonance Energy Transfer): investigation of macromolecular association and intermolecular distances; protein folding; energy harvesting; sensing.
Marcus theory and applications
- Nonlinear response theory and hyperpolarizabilities
- Second-order processes: second-harmonic generation and applications
- Third-order processes: Raman scattering, Two-Photon Absorption and their applications
- Confocal microscopy
- Multiphoton optical imaging
Optical Bloch equations and photon echo
Two-dimensional optical spectroscopy (IR and vis)
J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Springer 2006.
V. May, O. Kuhn, Charge and Energy Transfer Dynamics in Molecular Systems, Wiley 2004.
R. W. Boyd, Nonlinear Optics, Academic Press 2008.
Y. R. Shen, The Principles of Nonlinear Optics, Wiley-Interscience 1984.
P. Hamm and M. Zanni, Concepts and Methods of 2D Infrared Spectroscopy, Cambridge University Press 2011.
Classes and a couple of practical laboratory trainings
Assessment methods and criteria
Two options are possible: a classical exam (with questions on the course subjects) or the preparation of a report on a subjects of choice (related to the subjects of the course)