The course aims at giving a basic knowledge of techniques useful for studying biological macromolecules and problems of biophysical interest.
A part of the course gives some theoretical concepts of the interaction of light with proteins, necessary to comprehend some relevant experimental techniques, both at bulk and single molecule level. A second part of the course consists of practice in the lab where the students are learning how to perform measurements on their own.
The experimental techniques discussed are: steady-state and time resolved fluorescent spectroscopy, fluorescent correlation spectroscopy, photoacoustics and rapid mixing; outlines of one and two photons microscopy.
J.R. Lakovicz “Principles of fluorescence spectroscopy”, Kluwer Academic/Plenum Publishers, reviews and slides of lessons.
The exam will consist of writing a report for each experimental activity and a discussion on these reports.
Lessons and practice in the laboratory.