LABORATORY FOR BIOPHYSICS
Learning outcomes of the course unit
The course aims at giving a basic knowledge of techniques useful for studying biological macromolecules and problems of biophysical interest.
A part of the course gives some theoretical concepts of the interaction of light with proteins, necessary to comprehend some relevant experimental techniques, both at bulk and single molecule level. A second part of the course consists of practice in the lab where the students are learning how to perform measurements on their own.
Course contents summary
The experimental techniques discussed are: steady-state and time resolved fluorescent spectroscopy, fluorescent correlation spectroscopy, photoacoustics and rapid mixing; outlines of one and two photons microscopy.
J.R. Lakovicz “Principles of fluorescence spectroscopy”, Kluwer Academic/Plenum Publishers, reviews and slides of lessons.
Lessons and practice in the laboratory.
Assessment methods and criteria
The exam will consist of writing a report for each experimental activity and a discussion on these reports.