GENOMICS AND TRANSCRIPTOME ANALYSIS METHODS

What is a genome
Sequencing genomes
How to interpret information in a genomic sequence (in silico methods)
Genome anatomy
Transcription of complex genomes (human, animal, plant)
Synthesis and processing of RNA
Regulation of genome activity
Analysis of transcriptome
Molecular arrays and methods for analysis of arrays
Other methods for analysing the transcriptome (differential display, cDNA-AFLP, SAGE)
Quantification of the transcriptome
Methodologies in RealTime and theoretical bases
Genetic-molecular methods to study the function of individual genes (two hybrid, phage display, genetic footprinting, gene inactivation)
Site-specific mutagenesis
In vivo methods to analyse gene function in higher organisms (plant and animals)
Molecular methodologies to obtain knock-in, knock-out animals and mutagenised plants
Applications of functional genomics to pharmacology, medicine, agriculture, environment