GMO AND ANIMAL CELLULAR MODELS
This core module aims to introduce students to the concepts of ex vivo ed in vitro cell models used to study pathological processes involved in the major diseases.
-Organ and tissue culture
-Extracellular matrix and tissue dissociation
-Culture of animal cells
-Cell vitality and cell proliferation assays
-Organotypic culture models
Strategy of cell cultures
Biology of cultured cells.
Advantages and disadvantages of cell culture.
The environment of the cell culture: substrates, media, temperature, growth factors, etc.. "Scaling-up of cell cultures.
Kinetics of growth. Survival and toxicity.
Tissue dissociation and primary cultures.
Separation and characterization of specific cell types.
Maintenance of cultures.
Contamination of cell cultures.
The cancer cell: morphological and metabolic features.
Established cell lines.
Methods for transferring therapeutic genes
• transfection by physical methods: microinjection, electroporation, bombardment (gene gun)
• Lipofezione: liposomes, cationic lipid-DNA complexes
• viral vectors: retrovirus, adenovirus, adeno-associated virus, human herpesvirus.
R. Ian Freshney, Culture of Animal Cells: A Manual of Basic Technique, 5th Edition, ISBN: 0-471-45329-3 Ed Wiley-
Jan-Thorsten Schantz & Kee Woei Ng: A MANUAL FOR PRIMARY HUMAN CELL CULTURE - ISBN 981-238-796-X(pbk) ed. World Scientific
Alan Doyle , J. Bryan Griffiths: Cell and Tissue Culture: Laboratory Procedures in Biotechnology ISBN: 0-471-98255-5- Ed Wiley
Interactive oral lesson. Iconographic support will be provided.