HUMAN AND MODEL ORGANISMS MOLECULAR GENETICS GENOTOXICITY
Learning outcomes of the course unit
Aim of the course is to provide a panoramic view of the various aspect of genotoxicity and in particular of the environmental genotoxic risk at which humans are subjected. The course provides a detailed overview of methodologies for monitoring human populations for signs of exposure to genotoxic agents, and monitoring the environment itself (e.g., air, water, soil).
Course contents summary
Human genome Instability and DNA repair: Molecular basis of DNA alterations - Spontaneous alterations of and damages to DNA - Molecular mechanisms involved in the control of genome integrity - DNA repair in prokaryotic and eukaryotic organisms
Genotoxic agents: chemical and physical mutagens – Drug metabolizing enzymes – Antimutagens
Phenotypic effects of somatic mutations – Cancer genetics: cancer induction and progression pathways, genome stability.
Sources of genotoxic pollutants: natural and manmade mutagens and cancerogens in: environmental samples (air, soil, water, etc), diet and drugs.
Mutagenicity testing: biomarkers and biomonitoring; in vitro and in vivo mutagenicity tests.
Mutagenesi ambientale a cura di Lucia Migliore Ed. Zanichelli 2004, ISBN 8808-07719-5.
Tossicologia a cura di Greim H. e Deml E., Ed. Zanichelli 2000, ISBN 8808-09979-2.