Learning outcomes of the course unit
The objective of the course is to understand the basic concepts of enzymology such as: chemical and enzymatic kinetics, transition state theory, enzyme reaction types, enzymes, single and multi-layered enzymes, enzyme tests. The course also aims to introduce students to the possible industrial and/or medical applications where enzymes play a decisive role.
At the end of the course the student should have a clear understanding of the theory underlying enzymatic activity and a clear vision of biocatalytic applications in the world of work.
Course contents summary
- Brief history of enzymology
- Transition state theory
- Chemical and enzymatic catalysis
- Examples of enzymes
- Enzymatic kinetics
- Factors which influence enzymatic reactions
- Enzymatic inhibition
- Multi-layered kinetics
- Industrial applications
- Immobilised enzymes
- Protein engineering
ENZYMES, a Practical Introduction to Structure, Mechanism, and Data Analysis, SECOND EDITINO, Robert A. Copeland.
Fersht, A "Struttura e meccanismi d'azione degli enzimi" Ed. Zanichelli.