Learning outcomes of the course unit
Biochemistry is a research-based area of study. Study of basic aspects and application of methods and various techniques used in biochemical research.
Before taking the Applied Biochemistry exam, the Organic Chemistry exam must have been passed.
Course contents summary
General criteria in biochemical experimentation. Biological materials. Methods of separation and purification of proteins and other molecules of biochemical interest: centrifugation, salting out, dialysis, chromatography,electrophoresis. Biochemical applications of visible and ultraviolet spectroscopy. Study of enzymatic activity. Immunochemical, radioisotope and radioimmunologic techniques. Potentiometric and polarographic techniques Overview of molecular biology.
K.Wilson,J.Walker:''Biochimica e Biologia molecolare. Principi e tecniche'';
R.Reed,D.Holmes,J.Weyers:''Metodologie di base per le Scienze biomolecolari'';C.De Marco,C.Cini:”Principi di metodologia biochimica”.
Assessment methods and criteria