CLINICAL BIOCHEMISTRY DIAGNOSTIC TECHNIQUES
Learning outcomes of the course unit
The aim of this course is to provide the cultural basis for understanding the basic mechanisms both of the techniques commonly employed in the laboratory of clinical biochemistry (routine analysis) and some examples of more advanced test, found only in specialized laboratories.
Besides the obligatory prerequisites stated by the course of degree, Clinical Biochemistry is an interdisciplinary science and needs the basic knowledge in Chemistry, Biochemistry and Physiology.
Course contents summary
Analysis by the clinical laboratory: definition, typology, request.
Biological samples: collection, treatment and storage.
Instrument calibration; internal quality control; external quality control. Evaluation of the analytical performance.
Methods in the quantitative analysis of markers (function or damage) of liver, kidney, heart and skeletal muscle.
Urinalysis. Proteinuria and micro-proteinuria.
Analysis of plasma and urinary proteins: nephelometry, electrophoresis and immuno-fixation, capillary electrophoresis.
Enzyme determination in clinical chemistry. Activity or mass assay.
Lipids and dislipoproteinemia: principles and methods of analysis
Carbohydrates: methods for glycemia quantification.
The clinical laboratory in the autoimmune diagnostics. Immuno-enzymatic approach; principles and measure of a fluoroscopic preparation.
The clinical laboratory in the allergologic diagnostics.
A.Burlina. Medicina di laboratorio. Fondamenti di diagnostica -C.G. Edizioni medico scientifiche S.R.L.