APPLIED BIOCHEMISTRY AND MOLECULAR BIOLOGY
cod. 23840

Academic year 2007/08
2° year of course - First semester
Professor
Academic discipline
Biochimica clinica e biologia molecolare clinica (BIO/12)
Field
Formazione interdisciplinare
Type of training activity
Related/supplementary
40 hours
of face-to-face activities
4.5 credits
hub: -
course unit
in - - -

Integrated course unit module: APPLIED BIOCHEMISTRY AND MOLECULAR BIOLOGY

Learning objectives

<br />The main objective of the course  is to provide the students with the fundamentals of molecular biology, cellular biology and applied biochemistry, focusing on the basic concepts and applications related to the veterinary medicine. By the end of the course the student should be able to describe the mechanisms involved in the flow of the genetic information, in the biochemical regulation of the cellular functions, as well as the main issues regarding animal transgenesis and cloning. Furthermore the student should acquire the knowledge of the most important biochemical and molecular biology techniques applied in veterinary diagnostic. Finally he should demonstrate a critical comprehension of the molecular mechanisms involved in the regulation of the cell functions and their alteration in disease.

Prerequisites

General and organic chemistry, biochemistry

Course unit content

<br /><br />Molecular biology <br />The flow of the genetic information:<br />Nucleic acids: DNA and RNA. Genes, chromosomes and chromatin. Duplication and maintenance of genomic DNA. DNA transcription in prokaryotic and eukaryotic cells. Regulation of gene expression in prokaryotic and eukaryotic cells. Protein synthesis.<br />Fundamentals of molecular biology: recombinant DNA and gene cloning. Polymerase chain reaction (PCR and RT-PCR), Southern and Northern blotting<br />Applications of the molecular diagnostics and of the recombinant biotechnologies to the diagnosis and prevention of diseases. <br />Genetic manipulation in animals: animal transgenesis and animal cloning.<br /><br /><br /><br />Cellular biochemistry:<br />Cell structure and function. The nucleus. Protein sorting and transport. The cytoskeleton and cell movement. The cell surface. The extracellular matrix.<br />-Cell regulation: <br />The cell cycle. Cell signalling. Hormonal regulation and integration of metabolism.<br /><br /><br />Applied biochemistry<br />Electrophoresis, Western blotting, chromatography,   spectrophotometry, cell culture, proteomic and genomic.

Full programme

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Bibliography

<br />NELSON D.L., COX M.M: I principi di Biochimica di Lehninger, Zanichelli (2002)<br />COOPER G.M.: La cellula - un approccio molecolare, Zanichelli (1998)<br />ƒnLODISH H, BERK A, ZIPURSKY SL, MATSUDAIRA P, BALTIMORE D, DARNELL JE : Biologia<br />molecolare della cellula, Zanichelli (2002)<br />Alberts B.,Bray D., Hopkin K., Johnson A., Lewis J., Raff M., Roberts K., Walter P.: L¡¦essenziale di biologia molecolare della cellule, Zanichelli (2005)

Teaching methods

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Assessment methods and criteria

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Other information

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