CLINICAL MICROBIOLOGY DIAGNOSTIC TECHNIQUES
Learning outcomes of the course unit
To give the basic knowledge for the procedures and techniques used to demonstrate human parasitic infections.
Prerequisites
examinations of microbiology for the first and the second year s of the course.
Course contents summary
Techniques to manipulate biological samples for the parasitological examination
faecal samples
techniques for the microscopic examination (wet mount, concentration techniques, permanent staining – trichromic staining and modified Zehel-Neelsen staining
techniques for in vitro cultivation of intestinal protozoa
techniques for the culture-concentration procedure for larvae of intestinal nematoda
techniques for the detection of antigens of intestinal protozoa
techniques for the detection of nucleic acids of intestinal protozoa
blood samples
techniques for the microscopic examination (wet mount, concentration techniques, permanent and non permanent staining, Giemsa staining) for protozoa and elmints infecting blood and tissues
techniques for the detection of antigens of protozoa and elmints (microfilariae) infecting blood and tissues
techniques for the detection of nucleic acids of protozoa infecting blood
urine samples
techniques for the microscopic examination (wet mount, concentration techniques)
techniques for in vitro cultivation of protozoa infecting the urinary and genital tract
serum samples
techniques to detect specific antibodies to parasites by immunoassay, haemoagglutination assay, western blot assay.
Recommended readings
Diagnostic medical parasitology. Garcia and Bruckner . ASM ed.
Diangostic procedures listed in the protocols used during practical skills course.
