Learning outcomes of the course unit
In-depth insights of qualifying aspects instrumental to the acquisition of more specific expertise for the professional approach to virological diagnosis.
Course contents summary
In vitro cultivation of viruses: diploid and continuous cellular lines, preparation of cellular substrates. Virus-induced cytophatic effect: definition, causes, examples, identification. Immunofluorescence reaction: direct, indirect. Research and identification of viral proteins. Transmission electron microscopy: general principles, preparation of grids, negative staining, virus observation.
M. Bendinelli, C. Chezzi,, G. Dettori, N. Manca, G. Morace, L. Polonelli, M.A. Tufano. Microbiologia Medica. Monduzzi Editore.
Electron microscopy of viruses and virus-cell interactions.
Methods Cell Biol. 2008;88:497-524.
Hazelton PR, Gelderblom HR.
Electron microscopy for rapid diagnosis of infectious agents in emergent
Emerg Infect Dis. 2003 Mar;9(3):294-303.