Learning outcomes of the course unit
The module has as its formative aspective to provide a knowledge astructural and functional principles of molecules of biologicalinterest, and a general knowledge on fundamental metabolic pathways,their connexions and regulations.
Course contents summary
Enzymes - Kinetics of enzymatic reactions. The Michaelis-Menten constant (Km). Classification. Coenzymes. Isoenzymes. Competitive and non-competitive inhibition of enzyme activity. Allosteric enzymes.
Hemoglobin - Structure and function. An outline about synthesis and destruction.
Hormones - Overview and mechanisms of signal transduction.
Vitamins and coenzymes - Water-soluble vitamins: Thiamine (B1) - Riboflavin (B2) – Pyridoxine (B6) – Folic acid – Cobalamin (B12) – Biotin (H) – Pantothenic acid – Nicotinamide – Ascorbic acid. Lipid-soluble vitamins: Vitamin A – Vitamin D – Vitamin E – Vitamin K.
Coenzymes involved in hydrogen or electrons transfer & coenzymes involved in groups transfer.
Bioenergetics - Energy production and energy storage in the living cell. Some basic principles of bioenergetics. High-energy compounds.
Introduction to the metabolism - Catabolism. Anabolism. General aspects of metabolism regulation. Carbohydrates metabolism: digestion and absorption of carbohydrates. Glyolysis. Synthesis and catabolism of glycogen. The pentose pathway. The Krebs citric acid cycle. Gluconeogenesis. Lipid metabolism: digestion and absorption of fat. Fatty acids b-oxidation. Synthesis and oxidation of ketone bodies. Biosynthesis of fatty acids. Structure and function of cholesterol. Amino acid metabolism: digestion of proteins and absorption of amino acids. Essential and non-essential aminoacids. Main reactions of amino acids: deamination and transamination. Fate of the amino group and ammonia. The urea cycle. Decarboxylation.
P.C. Champe et al. “Le basi di biochimica”, Zanichelli Editore.
M.V. Catani et al. “Appunti di Biochimica”; Piccin Editore.
oral lesson with slides
written tests (quiz)