MICROBIOLOGY
Course unit partition: PIACENZA

Academic year 2008/09
1° year of course -
Professor
Academic discipline
Microbiologia e microbiologia clinica (MED/07)
Field
Ambito aggregato per crediti di sede
Type of training activity
Base
10 hours
of face-to-face activities
1 credits
hub: PIACENZA
course unit
in - - -

Course unit partition: MICROBIOLOGY

Learning objectives

 <br />Acquisition of the essential knowledge about the general characters of etiologic agents of infectious diseases (bacteria, fungi, helminths, protozoa, viruses), host-etiological agents interactions, prophylaxis and therapy (notes), drug-resistance mechanisms, nosocomial infections.<br /> 

Prerequisites

To pass the exams of the first semester, first year.

Course unit content

<br />
<br />
 Bacteriology<br />
General characters of prokaryotic cell<br />
         Morphology of prokaryotic cell<br />
         Prokaryotic cell structures (cell wall, cytoplasmic membrane, nucleus, flagella, pili and          fimbriae, capsule, endospores)<br />
         Notes on bacterial metabolism and replication<br />
Microbial pathogenicity<br />
         Host-microbe interactions<br />
         Normal flora of humans<br />
         Microbial pathogenicity and potential virulence factors (adhesins, enzymes, bacterial          toxins)<br />
Laboratory diagnosis of bacterial diseases<br />
         Bacterial (components) detection and identification. Notes on laboratory methods (sample          collection and transport, microscopic examination, cultural examination, bacterial          identification, molecular biology approaches)<br />
         Antibody detection (notes)<br />
Antibiotics and synthetic antimicrobial agents; methods for antibacterial susceptibility testing (notes)<br />
Important pathogenic bacteria (notes)<br />
-          Staphylococci (S. aureus and coagulase-negative staphylococci)<br />
-          Streptococci (Group A beta-haemolitic streptococci, Pneumococci)<br />
-          Neisseria meningitidis and N. gonorrhoeae<br />
-          Corynebacterium diphtheriae<br />
-          Mycobacteria<br />
-          Enteropathogenic Gram-negative bacteria: Salmonella, Shigella, Yersinia, Campylobacter and Vibrio<br />
-          Clostridia (C. tetani, C. perfringens and C. botulinum)<br />
-          Gram-negative and Gram-positive bacteria causing nosocomial infections: Pseudomonas and S. aureus.<br />
 <br />
Mycology<br />
General characters of fungi<br />
The fungal cell, the fungal colony; Yeasts and moulds<br />
Classification of mycoses; Common human mycoses<br />
Antifungal agents<br />
 <br />
Parasitology<br />
Protozoa: Lyfe cicles - notes<br />
-Intestinal parasites:<br />
 Giardia intestinalis<br />
 Cryptosporidium<br />
 Entamoeba histolytica<br />
-Tissues and blood parasites:<br />
 Toxoplasma gondii<br />
 Plasmodium<br />
 Leishmania<br />
 Free living amebas (Acanthamoeba)<br />
- Uro-genital tract parasites:<br />
 Trichomonas vaginalis<br />
Helminths: Lyfe cicles - notes<br />
- Cestodes:<br />
 Taenia solium<br />
 Taenia saginata<br />
 Diphyllobotrium latum<br />
 Echinococcus<br />
- Nematodes:<br />
 Enterobius vermicularis <br />
 Ascaris lumbricoides<br />
 Strongyloides stercoralis<br />
 Trichinella spiralis<br />
Arthropods: Lyfe cicles - notes<br />
 Sarcoptes scabiei<br />
 Pediculus humanus var. corporis and var. capitis<br />
 <br />
Virology<br />
Morphological and structural characteristics of viruses<br />
         Definition<br />
         Size and morphology<br />
         Chemical composition<br />
         Nucleic acids<br />
Classification of viruses (notes)<br />
Viral replicative cycle<br />
         Attachment<br />
         Entry<br />
         Uncoating<br />
         Genome expression and replication<br />
Virus cultivation<br />
         Laboratory animals, embryonated chicken eggs, cell cultures <br />
         Cytopathic effects<br />
Laboratory diagnosis of viral infection<br />
         Clinical samples (collection, transport and storage criteria)<br />
         Virus (components) detection (cytologic evaluation, viral culture, rapid antigen detection,          gene amplification)<br />
         Antibody detection techniques<br />
Common human viral diseases

Full programme

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Bibliography

 - M. Bendinelli, C. Chezzi, G. Dettori, N. Manca, G. Morace, L. Polonelli, M.A. Tufano - <br />
"MICROBIOLOGIA MEDICA: BATTERIOLOGIA, MICOLOGIA, VIROLOGIA. Gli agenti infettivi dell'uomo: biologia, azione patogena, laboratorio. " Monduzzi Editore <br />
- I. De Carneri - Parassitologia Generale ed Umana, Casa Editrice Ambrosiana, Milano. <br />
<br />

Teaching methods

Teacher: Prof. Giuseppe Dettori.

Assessment methods and criteria

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Other information

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