CELLULAR MODELS AND GMOs
Within the scope of the integrated course, the unit aims to provide students with elements and cultural tools for setting up suitable ex vivo and in vitro models for studying physiopathological mechanisms of diseases.
The course will develop with classroom lectures that will discuss the following topics:
Organ and tissue cultures
The extracellular matrix and tissue dissociation
Culture of animal cells
Transfer of gene material
Assessment of cell viability and proliferation
Assessment of cytotoxicity
R. Ian Freshney, Culture of Animal Cells: A Manual of Basic Technique, 5th Edition, ISBN: 0-471-45329-3 Ed Wiley-
Jan-Thorsten Schantz & Kee Woei Ng: A MANUAL FOR PRIMARY HUMAN CELL CULTURE - ISBN 981-238-796-X(pbk) ed. World Scientific
Alan Doyle , J. Bryan Griffiths: Cell and Tissue Culture: Laboratory Procedures in Biotechnology ISBN: 0-471-98255-5- Ed Wiley
Classroom lectures with the use of audiovisual aids, discussion in the classroom with the students, individual conversations on request, practical activities in the cell culture laboratory.
Oral test according to the examination timetable of the Integrated Course.