MICROBIAL GENOMICS - REVERSE GENETICS
Learning outcomes of the course unit
Aims of the course is the study of genetic approaches applied to genomics and functional analysis in microorganisms. Particular attentions will be paied to yeast, a model organism in fundamental and biotechnologic applications.
Course contents summary
Forward and reverse genetics. Functional analysis in yeast: cloning of yeast gene by functional complementation; functional analysis by construction of gene deletion mutants; analysis of essential genes; yeast genomics.
Gene products interaction: multicopy suppressors; synthetic letals.
Yeast as a model organism in the study of mitochondrial genetics: maternal genetics; petite mutants; nuclear and mitochondrial pet mutants; mitochondrial genome organization; mitochondrial biogenesis.
Yeast as a model organism in the study of mitochondrial diseases
Metabolic engeneering in yeast and other microorganisms
Methods for functional analysis in other organisms: in Drosophila, in plants
Original papers selected by the teacher