Learning outcomes of the course unit
The module has as its formative aspective to provide a knowledge astructural and functional principles of molecules of biologicalinterest, and a general knowledge on fundamental metabolic pathways,their connexions and regulations.
Course contents summary
Enzymes - Kinetics of enzymatic reactions. The Michaelis-Menten constant (Km). Classification. Coenzymes. Isoenzymes. Competitive and non-competitive inhibition of enzyme activity. Allosteric enzymes.
Hemoglobin - Structure and function. An outline about synthesis and destruction.
Hormones - Overview and mechanisms of signal transduction.
Vitamins and coenzymes - Water-soluble vitamins: Thiamine (B1) - Riboflavin (B2) – Pyridoxine (B6) – Folic acid – Cobalamin (B12) – Biotin (H) – Pantothenic acid – Nicotinamide – Ascorbic acid. Lipid-soluble vitamins: Vitamin A – Vitamin D – Vitamin E – Vitamin K.
Coenzymes involved in hydrogen or electrons transfer & coenzymes involved in groups transfer.
Bioenergetics - Energy production and energy storage in the living cell. Some basic principles of bioenergetics. High-energy compounds.
Introduction to the metabolism - Catabolism. Anabolism. General aspects of metabolism regulation. Carbohydrates metabolism: digestion and absorption of carbohydrates. Glyolysis. Synthesis and catabolism of glycogen. The pentose pathway. The Krebs citric acid cycle. Gluconeogenesis. Lipid metabolism: digestion and absorption of fat. Fatty acids b-oxidation. Synthesis and oxidation of ketone bodies. Biosynthesis of fatty acids. Structure and function of cholesterol. Amino acid metabolism: digestion of proteins and absorption of amino acids. Essential and non-essential aminoacids. Main reactions of amino acids: deamination and transamination. Fate of the amino group and ammonia. The urea cycle. Decarboxylation.
Giuseppe Arienti. “Un compendio di Biochimica”; Piccin Editore.
oral lesson with slides
written tests (quiz)