LABORATORY FOR PHYSIOLOGY
Learning outcomes of the course unit
To acquire expertise in the use of modern approaches for measuring and processing data relating to cardiac electro-mechanical function
Elements of Biochemistry, Molecula Biology and General Physiology
Course contents summary
- Measurements of the electro-mechanical competence of the heart at different levels of complexity, from the organ to cellular/molecular level.
- Analysis of changes induced by different heart conditions on cardiac electrical and mechanical properties with focus on the underlying molecular mechanisms.
- Cardiac electrical activity: ECG telemetry recording in conscious freely moving animals; measurement of tissue eletrophysiological properties by simultaneously recording multiple epicardial electrograms from isolated or “in situ” hearts; measurement of cellular electrophysiological properties in enzimatically isolated cardiomyocytes (patch-clamp).
- Cardiac mechanical activity: recording of hemodynamic parameters in intact animals; measurement of cellular mechanical properties and calcium transients in enzimatically isolated cardiomyocytes
- Morphometric and immunohistochemical analyses on myocardial tissue samples.
- Molecular analyses (Western blot, RT-PCR) to evaluate the expression levels of protein involved in cardiac excitability, conduction and contraction.
Published papers provided by the teacher.
Classroom lectures and laboratory experiences on animal models
Assessment methods and criteria
Written test on the laboratory experience and seminars