Learning outcomes of the course unit
Aim of the course is to provide a panoramic view of the various aspect of the environmental mutagenesis and in particular of the environmental genotoxic risk at which human and animals are subjected. The course provides a detailed overview of methodologies for monitoring human populations for signs of exposure to genotoxic agents, and monitoring the environment itself (e.g., air, water, soil).
Basic knowledge of Genetics, Biochemistry and Molecular Biology
Course contents summary
Molecular basis of DNA alterations
spontaneous alterations of and damages to DNA
chemical and physical mutagens
molecular mechanisms of mutagenesis
Molecular mechanisms involved in the control of genome integrity
metabolism of xenobiotics
DNA repair in prokaryotic and eukariotic organisms
Mutations in somatic cells
mutagenesis and cancerogenesis
Mutations in germ cells
Sources of genotoxic pollutants
natural and manmade mutagens and cancerogens in: environmental samples (air, soil, water, etc.) and diet
methods and testing strategies: in vitro and in vivo mutagenicity tests.
Protocols for genotoxins assessment
environmental mixtures and biological samples
guidelines for mutagenicity testing and risk assessment.
Mutagenesi ambientale a cura di Lucia Migliore Ed. Zanichelli 2004, ISBN 8808-07719-5.
Tossicologia a cura di Greim H. e Deml E., Ed. Zanichelli 2000, ISBN 8808-09979-2.