Learning outcomes of the course unit
The first part of the course will provide a systematic presentation of the main techniques used in biochemistry laboratories. The second part of the course will offer an introduction to proteomics, to its experimental approaches and to the research areas typical of this discipline.
Pre-requisites (strongly recommended): basic courses in Physics, Chemistry, Microbiology and Biochemistry.
Course contents summary
Part 1. Basic biochemistry methodologies
Protein extraction and purification.
Electrophoretic techniques and a brief outline of chromatographic techniques.
Immunochemical techniques and their applications.
Use of radioisotopes in biochemical research.
Enzyme kinetics and enzyme assays.
Part 2. Proteomics
Main directions in post-genomic research. Systematic proteomics, differential proteomics and functional proteomics.
Methodological basis for proteomic approach: bi-dimensional protein electrophoresis; mass spectrometry coupled with database analyses.
Analysis of some exemplar proteomic studies aimed at addressing biological problems.
For the course section dealing with basic biochemical methodologies, a concise but useful text is:
Reed, Holmes, Weyers & Jones, Metodologie di base per le scienze biomolecolari, Zanichelli
For the proteomics section, there are no textbooks but most of the lessons will be based on scientific articles (in English) made available to the students.
The course is constituted by formal classroom presentations.
The final assessment of the students will be based on a written test.