ANALYTICAL CHEMISTRY B LABORATORY (UNIT 1)
Learning outcomes of the course unit
To provide the fundamental concept of instrumental analysis.
At the end of the course, for a given analytical problem, the students should be able to point out the most suitable analytical technique to solve it
Basic knowledge of general and organic chemistry
Must be attended after "Chimica Analitica A"
Course contents summary
The interaction light-matter. Spectral ranges of analytical concern and types of transitions.
UV-Vis molecular absorption molecular spectrometry: spectra origin; relationship between electronic structure and absorption bands; principles of instrumentation; quantitative analysis.
IR and Near-IR molecular absorption spectrometry: spectra origin; principles of instrumentation and main applications.
Atomic emission and absorption spectometries: spectra origin; principles of instrumentation; atomic sources; atomisation systems; background correction systems; application to determinations of elements (metals) at very low level of concentration; sample treatment.
Liquid chromatography: Separative mechanisms using liquid and solid stationary phases; classification of chromatographic separation mechanisms: adsorption, repartition, ion exchange, size exclusion; principles of HPLC instrumentation; column, pump and detector classification. Main environmental applications.
Gaschromatography: interaction mechanisms; classification of stationary phases and various substance according to their polarity; capillary and packed columns; principles of instrumentation: types of injectors, columns and detectors; sampling methods for volatile substances; dynamic and static headspace techniques; Main environmental applications.
Scope and peculiarity of the analysis; principles of instrumentation: ion sources, mass analysers (magnetic field, quadrupolar etc); information provided by the mass spectrum; fragmentation; Coupling of the mass spectrometer with the GC and other hyphenated techniques; environmental applications.
D. C. Harris, Chimica Analitica Quantitativa - Zanichelli
D. Skoog, J. Leary, Chimica Analitica Strumentale - EdiSES
K.A. Rubinson, J.F. Rubinson, Chimica Analitica Strumentale - Zanichelli
R. Kellner J.M. Mermet, M. Otto, H.M. Widmer (Eds), Analytical Chemistry - Wiley-VCH (ISBN 3-527-28881-3)
The course is integrated with 'Chimica Analitica A' (first semester of second year).
Written and oral examinations concerning both modules (A and B) and the laboratory exercitations