Learning outcomes of the course unit
This course will provide students with a systematic knowledge of the main techniques used in biochemical laboratories and an introduction to proteomics, the research areas typical of this discipline and the experimental approaches used.
It is strongly recommended to have done basic courses in Physics, Chemistry, Microbiology and Biochemistry.
Course contents summary
Part 1. Basic biochemistry methodologies
Protein extraction and purification.
Electrophoretic techniques and a brief outline of chromatographic techniques.
Immunochemical techniques and their applications.
Use of radioisotopes in biochemical analyses.
Enzymatic reporter system and activity units.
Part 2. Proteomics
Main directions in post-genomic research: proteomics and functional genomics.
Methodological basis for proteomic approach: protein mono- and bi-dimensional electrophoresis; mass spectrometry coupled with database analyses.
Detailed examination of some examples of particular importance in proteomic approaches to the study of biological problems.
For the part on basic biochemical methodologies, a concise but useful text is:
Reed, Holmes, Weyers & Jones, Metodologie di base per le scienze biomolecolari, Zanichelli
For the part on proteomics, there are no textbooks but most of the lessons will be based on scientific articles (in English) placed at the disposal of students.
The course will be in lecture form.
Final evaluation will be based on a written test.