DRUG ANALYSIS II
Learning outcomes of the course unit
The course is divided into theoretical lectures and practical exercises and has been designed to provide students with knowledge on the most useful analysis methods for separation, identification and purity control of substances of pharmaceutical interest.
Students should have passed the Organic Chemistry examination to attend the Drug Analysis II laboratory
Course contents summary
Safety rules and elements of first aid in the chemical-pharmaceutical laboratory .
Explanation and use of the Official Italian Pharmacopoeia and of the European Pharmacopoeia.
Drug isolation and purification techniques: extraction using solvents, crystallisation, distillation, sublimation, chromatographic methods.
Measurement of physical constants: boiling point, fusion point, specific rotatory power.
Chromatographic techniques applied to drug recognition.
Spectrophotometric methods for drug identification
Identification of organic substances:
preliminary tests: organoleptic examination, behaviour during combustion, solubility, qualitative elementary analysis;
recognition assays of the main functional groups and preparation of crystalline derivatives (amines, carbonyl compounds, carboxylic acids, alcohols, phenols, nitro compounds, carbohydrates, amino acids).
Recognition assays of organometallic and inorganic substances of pharmaceutical interest.
Recognition of organic, organometallic and inorganic substances included in the Official Pharmacopoeia: identification reactions and purity control. Limit assays.
- F. SAVELLI, O. BRUNO: “Analisi Chimico Farmaceutica” – PICCIN
- F. CHIMENTI: "Identificazione sistematica di composti organici" - Grasso, Bologna
- O. LIVI, A. BALSAMO: Guida pratica al riconoscimento delle sostanze iscritte nella X edizione della Farmacopea Ufficiale della Repubblica Italiana" - Edizioni ETS, Pisa.
- F. SAVELLI, A. BOIDO: "Guida all'analisi di composti di interesse farmaceutico" - ECIG, Genova.
- A. VOGEL: "Chimica organica pratica" - Ambrosiana Editrice, Milano.
- European Pharmacopoeia.
The courses is divided into theoretical lectures and practical activities.
The examination consists of a practical test (recognition of two substances included in the Official Pharmacopoeia), a written test and an oral test covering all the topics of the course syllabus.