Learning outcomes of the course unit
The course provides basic notions for the qualitative and quantitative determination of substances of pharmaceutical interest. In particular, during the practical training the attention will be focused on the qualitative analysis of inorganic salts and on the quantitative determination of inorganic and organic analytes.
Prerequisite course: General and Inorganic Chemistry
Course contents summary
Safety and first aid procedures in the chemical and pharmaceutical laboratory. Symbols, risk and safety phrases. Hazard chemicals handling procedures.
Lab equipment and instrumentation: the analytical balance, burettes, pipettes, the pH-meter, calibration of measuring devices.
Basic laboratory techniques and operations: solubilisation, dilution, concentration, desiccation, precipitation, decantation, filtration, centrifugation, solvent extraction.
Introduction to Qualitative Inorganic Analysis. Preliminary Tests. Solubility.
Combustion assay, flame test.
Qualitative analysis in aqueous solution: reactions of main anions and cations with particular reference to inorganic or organo-metallic compounds enrolled in the Official European Pharmacopeia, analysis of acid insoluble residual.
Introduction to Quantitative Pharmaceutical Analysis. Sampling. Experimental error: types of errors, precision and accuracy, expression and propagation of uncertainty. Standard and normal solutions. Volumetric and gravimetric analysis.
Alkalimetry and acidimetry: preparation and standardization of solutions.
Analysis of the titration curve: choice of the indicator, potentiometric determination of the final point.
- ARANEO: “Chimica analitica qualitativa. Nuovo metodo periodale”, Ambrosiana Ed.
- BARBETTI P., QUAGLIA M.G. “L’analisi qualitativa in chimica farmaceutica e tossicologica inorganica”, Galeno Ed.
- HOGNESS, JOHNSON, ARMSTRONG “Analisi qualitativa ed equilibrio chimico”, Piccin Ed.
- HARRIS D.C., “Chimica Analitica Quantitativa”, Zanichelli Ed
The exam will consist in practical, written and oral tests. Practical exercitations during the course will be taken into due consideration