APPLIED BIOCHEMISTRY
cod. 00062

Academic year 2008/09
2° year of course - Second semester
Professor
Academic discipline
Biochimica (BIO/10)
Field
Discipline biologiche e farmacologiche
Type of training activity
Characterising
40 hours
of face-to-face activities
5 credits
hub: -
course unit
in - - -

Integrated course unit module: BIOCHEMISTRY - APPLIED BIOCHEMISTRY

Learning objectives

Biochemistry is a research-based area of study. Study of basic aspects and application of methods and various techniques used in biochemical research.

Prerequisites

Before taking the Applied Biochemistry exam, the Organic Chemistry exam must have been passed.

Course unit content

<br />General criteria in biochemical experimentation. Biological materials. Methods of separation and purification of proteins and other molecules of biochemical interest: centrifugation, salting out, dialysis, chromatography,electrophoresis. Biochemical applications of visible and ultraviolet spectroscopy. Study of enzymatic activity. Immunochemical, radioisotope and radioimmunologic techniques. Potentiometric and polarographic techniques Overview of molecular biology.

Full programme

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Bibliography

<br />
K.Wilson,J.Walker:''Biochimica e Biologia molecolare.Principi e tecniche''<br />
R.Reed,D.Holmes,J.Weyers:''Metodologie di base per le Scienze biomolecolari''

Teaching methods

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Assessment methods and criteria

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Other information

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