BIOINORGANIC CHEMISTRY AND BIOCRYSTALLOGRAPHY
Learning outcomes of the course unit
This course has the purpose to provide the students with those knowledges that will allow them to read critically papers concerning bioinorganic issues and to join with little difficulties research groups dealing with bioinorganic chemistry.
Basics of Coordination Chemistry and Biochemistry are advisable.
Course contents summary
The cycles of the main elements involved in the functioning of living organisms – Summary of the main metalloenzymes and metalloproteins studied in the course – Proteins and nucleic acids from a structural perspective – Protein crystallography: preparing crystals, preliminary characterization, reciprocal lattice, data collection, solution of the phase problem, refinement and structure - Protein data bank and use of RASMOL to display proteins - Roles of metalloproteins in cells: choice, uptake and assembly of metal containing units in biology – Control and use of of ion concentration in the cell - Influence of metals in folding and cross linking in biomolecules – Interactions between metal ions and complexes in biomolecules – Electron transport proteins _ Nonredox activation mechanisms and interactions with substrates – Atom and atom groups transfer chemistry – Tuning of metal properties by proteins to obtain specific functions Metal protein analysis according to the metal: Iron, Copper, Molybdenum, Cobalt, Zinc and other metals. Metals in medicine.
S J Lippard, J M Berg. 1994. Principles of Bioinorganic Chemistry. University Science Books Mill Valley, California
R. M. Roat-Malone. 2002. Bioinorganic Chemistry: A Short Course. John Wiley & Sons, New Jersey, USA.
W Kaim, B Schwederski. 1995. Bioinorganic Chemistry. John Wiley & Sons, New York
D.E. McRee. 1999. Practical Protein Crystallography. Academic Press. San Diego
Lectures supported by Power Point slides.
The examination consists in a presentation of a bioinorganic paper assigned to the student followed by more general questions about the main subjects presented in the course.