APPLIED BIOCHEMISTRY AND MOLECULAR BIOLOGY(B)
Learning outcomes of the course unit
The main objective of the course is to provide the students with the fundamentals of molecular biology, cellular biology and applied biochemistry, focusing on the basic concepts and applications related to the veterinary medicine. In particular, part of the course will be focused on the different aspect of protein synthesis and assembling of the cytoskeleton. The aim is of providing the students with the essential tools for the interpretation of the physiological and pathology mechanisms related to these biochemical processes. In addition, the students will be introduced to the basic techniques of cell cultures, immuno-diagnostics, Proteomics and to the applications of Stem cell biology in the field of Veterinary Medicine.
Course contents summary
A – Cellular Biochemistry: Signal peptide. The lndoplasmic reticulum. The Golgi apparatus. The lysosomes. The protein secretory pathway. The Cytoskeleton. The cellular movement. The biological molecular motors.
B – Applied Biochemistry: Production and applications of monoclonal antibodies (Hybridoma Technology, ELISA test, Western Blotting test for the identification of BSE, immunofluorescence microscopy, Confocal mycroscopy. Proteomics.
C – Cellular Biology: Stem cell biology. Introduction to cell therapy in the field of Veterinary Medicin
NELSON D.L., COX M.M: I principi di Biochimica di Lehninger, Zanichelli (2002)
COOPER G.M.: La cellula - un approccio molecolare, Zanichelli (1998)
ƒnLODISH H, BERK A, ZIPURSKY SL, MATSUDAIRA P, BALTIMORE D, DARNELL JE : Biologia
molecolare della cellula, Zanichelli (2002)
Alberts B.,Bray D., Hopkin K., Johnson A., Lewis J., Raff M., Roberts K., Walter P.: L¡¦essenziale di biologia molecolare della cellule, Zanichelli (2005)