DIAGNOSTIC TECHNIQUES OF CLINICAL MICROBIOLOGY
Learning outcomes of the course unit
The objectives of the course are to enable the acquisition of knowledge and understanding about basic technical aspects related to the laboratory diagnosis of infections caused by relevant bacteria, fungi, and viruses of medical interest. In addition, they must be able to apply this knowledge in the workplace.
Basic knowledge of biology, biochemistry and physiology are suitable to understand the course topics successfully.
Course contents summary
Basic microscopic and culture techniques for the laboratory diagnosis of bacterial, fungal and viral infections; main techniques for laboratory diagnosis of infection by the principal protozoa of medical interest.
Techniques for microscopic examination: simple and differential staining. Metabolism Cultivation techniques: liquid and agar media.
Techniques for the laboratory diagnosis of infection by the principal protozoa of medical interest: Plasmodium sp., Leishmania, Toxoplasma gondii, free-living amoebae, Entamoeba histolytica, Giardia intestinalis, Cryptosporidium spp.), Trichomonas vaginalis.
Techniques for microscopic examination and cultivation of yeasts and filamentous fungi.
Techniques for microscopic examination of viruses: electron microscopy; use of optical and fluorescence microscopy for viral infection laboratory diagnosis.
Cultivation of viruses: use of tissue cultures; cell culture (rapid and conventional methods).
Principles of laboratory diagnosis of microbial and viral infections.
Eudes Lanciotti - Microbiologia Clinica – Casa Editrice Ambrosiana;
Iconographic material used in class available on the Elly site, on the course page.
Lectures supported by visual aids will be used to present and discuss the contents of the course.
Assessment methods and criteria
Learning outcomes will be verified through an oral examination. The examination can be addressed, upon registration online, based on the official exams calendar of the Department. By questions regarding the course content it will be ascertained whether the student has achieved the objective of knowledge and understanding of the scientific discipline in order to access the following years of the bachelor course in TLB.. The ascertained inability to answer a question or to prove at least a basic knowledge of the subject will prevent the successful completion of the exam. In case of passing the exam, the attribution of the vote, expressed in thirtieths, will be carried out according to the degree of achievement of the objectives (excellent, good, fair, fully sufficient, barely sufficient). The exam score will contribute to the final vote of the integrated course of Microbiology and Clinical Microbiology I, obtained on the basis of the average of the notes obtained in the two modules. Full marks with laude will be reserved to students who have demonstrated particular communication and autonomous learning skills. The result of the examination is communicated immediately to the student, and, after acceptance, it will be published on the ESSE3 portal (https://unipr.esse3.cineca.it/Home.do).