GENETICS AND INTEGRATED BIOTECHNOLOGIES LABORATORY I
Learning outcomes of the course unit
To provide the first year students with the basic concepts of classical and molecular genetics
Course contents summary
The cours is composed of two main sections. 1.CLASSICAL GENETICS: Mitosis and meiosis. Mendelian genetics. Independent assortment and genetic characters segregation. Modification of mendelian ratios: Co-dominance, Multiple alleles, Lethal alleles, Gene interaction. X-linked genes. Chromosome theory of inheritance. Linkage and recombination. Crossing over. Recombination in yeast (tetrads analysis). Mechanisms of genetic recombination in prokaryotes: bacterial transformation, bacterial conjugation, transduction
2. MOLECULAR BASIS OF HEREDITY: Experiments leading to the discovery of DNA as genetic material. DNA: structure and replcation. Chromosome structure in prokaryotes and eukaryotes. RNA structure, transcription and translation. The genetic code and its deciphering. Gene structure and organisation. Interrupted genes. The one-gene: one-enzyme hypothesis. Dissection of biochemical pathways. Genetic complementation. Gene expression. Genome, chromosomes and gene mutations. Molecular basis of the mutation. Reversion. Suppression. Detection of spontaneous or induced mutants. Regulation of gene expression in prokaryotes. Operon Lac: negative and positive control.
Practical and theoric activities are supporting the lessons.
Snustad DP, Simmons MJ (2014)
Principi di Genetica
Binelli, Ghisotti (2018)
Griffiths AJF, Wessler SR, Carroll SB, Doebley J (2013)
Frontal lectures and exercises; laboratory activities
Assessment methods and criteria
Written and oral examen