DESIGN OF BIOTECHNOLOGICAL VACCINES
Learning outcomes of the course unit
The course aims to allow the student to know and understand basic notions and updated scientific knowledge about anti-infective vaccinology, relevant existing vaccines, biotechnological vaccines (characteristics and development), existing biotechnological tools in vaccinology, and provide the ability to use in occupational contexts the knowledge and understanding acquired in the field.
Course contents summary
Principles and immunological basis of vaccinology. Relevant anti-infective available or experimental vaccines. Biotechnological vaccines and their design. Rational design. Reverse vaccinology. Available biotechnological vaccines. Recombinant bacteriophges and bacteria as vaccines and vaccine delivery vectors. Plant-based vaccines. Main vaccine problems still not resolved. Routes of administration. Adjuvants and delivery systems.
Introduction: principles and immunological basis of vaccinology. Main available anti-infective vaccines: live-attenuated (smallpox, measles, mumps, rubella, varicella, typhoid fever, yellow fever), killed-inactivated (poliomyelitis, influenza, hepatitis A), anatoxins (tetanus, diphtheria, pertussis), subunits, purified antigens, conjugated, recombinant and experimental (DNA, anti-idiotypic). Experimental anti-mycotoxin vaccines. Biotechnological vaccines and their design. Main available biotechnological vaccines: HBV, HPV, Rotavirus, polysaccharide-conjugated vaccines (Haemophilus influenzae, Neisseria meningitidis, Streptococcus pneumoniae). Calendar of vaccinations in Italy. Rational design. Reverse vaccinology (anti-N. meningitidis group B vaccine). Recombinant bacteriophges and bacteria as vaccines and vaccine delivery vectors. Plant-based vaccines. Relevant unresolved issues: malaria, toxoplasmosis, ebola, HCV, HIV and others. Routes of administration. Adjuvants and delivery systems.
Iconographic material used during lectures. Reviews on specific topics. References on specific subjects provided during lectures.
The topics concerning the contents of the course will be presented and discussed through lectures with the help of iconographic material collected in PowerPoint files which, at the end of the course, will be made available to the students. During the lessons the main concepts of each topic will be exposed, emphasizing the links between the various parts of the course and encouraging the active participation of the students.
Assessment methods and criteria
The assessment of the achievement of the objectives foreseen by the course will be carried out through an oral examination. Questions on the topics relating to the course will be used to verify whether the student has achieved the objective of knowledge and understanding the scientific discipline and is able to use the technical-scientific language in an appropriate manner and to apply in occupational contexts the knowledge acquired. Failure to answer to one question, or proven inability to demonstrate at least a basic knowledge on the subject, will prevent the successful completion of the exam. Upon successful completion of the examination, a grade, expressed in thirtieths, will be attributed according to the degree of achievement of the objectives (excellent, good, fair, fully sufficient, barely sufficient). The honors will be given to students who have demonstrated particular communication skills and autonomous learning. The result of the examination is communicated immediately to the student, and, after its immediate acceptance, is then published on the ESSE3 portal (https://unipr.esse3.cineca.it/Home.do).