HUMAN PHYSIOLOGY AND CLINICAL BIOCHEMISTRY
Learning outcomes of the course unit
The course provides the information and the scientific supports for
a correct methodological approach that develops the knowledge and the
necessary understanding of the topics of Clinical Biochemistry. This is to allow a
correct approach to the practical issues which are specific to the student of the
Bachelor Degree in Techniques Of biomedical laboratory. The acquired
skills will allow to understand the complexity of biological samples, their
variability, and the critical steps associated with their treatment and
conservation. This will allow you to recognize the possible sources of
error and apply the appropriate remedies.
There is no mandatory propedeuticity, but considering that Clinical
Biochemistry is an interdisciplinary subject, the student should have the
basic concepts of Biology, Anatomy, Physiology, Chemistry and
Course contents summary
The course integrates Biochemistry,
Instrumental Biochemistry and General Clinical Biochemistry.
The use of laboratory data in clinical practice. The interpretation of
biochemical data. Reference and variability of laboratory data. Disorders
of the hydrosiline equilibrium and of the acid-base balance. Mechanisms underlying the general functions of the cells as transports, electrical and chemical communication, mechanical activity, metabolism.
Functions of the main apparatus of the body as cardiovascular, pulmonary, renal and endocrine.
Determination of enzymatic activity. Analysis of blood and urine.
Evaluation of hepatic function and diagnosis of
jaundice. Diabetes mellitus.
Glycemic homeostasis. Nucleotide metabolism and hyperuricemia.
Metabolism of amino acids in clinical biochemistry. Clinical Molecular
Biology and Molecular Diagnosis. Tumor markers.
1. Sacchetti L., Cavalcanti P., Fortunato G., Pastore L., Rossano F.,
Salvatore D., Scopacasa F. “Medicina di Laboratorio e Diagnostica
Genetica” Idelson-Gnocchi, Sorbona ISBN: 8879474537.
2. Giorgio Federici , Medicina di laboratorio 3/ed , McGraw-Hill, ISBN:
3. Denise D. Wilson, Manuale di Tecniche Diagnostiche ed Esami di
Laboratorio, McGraw-Hill, ISBN: 9788838639500.
4. William J. Marshall, Stephen K. Bangert, Clinical Biochemistry:
Metabolic and Clinical Aspects, Elsevier
5. Barbiroli B., Filadoro F., Branzini C., Sacchetti L., Salvatore F. “Medicina
di Laboratorio” Edizioni UTET.
6. Principi di biochimica Lehninger - Zanichelli
Assessment methods and criteria
The final exam is oral and focuses on the topics of the program.