Learning outcomes of the course unit
Educational objectives of the course are:
- knowledge and understanding of the main strategies and molecular genetics techniques used for the study of genes and genomes, for the recombinant production, for forensic analysis.
- the ability to apply knowledge to solve specific problems in the field of study
-autonomy of judgment, i.e. the ability to evaluate and to interpret experimental data
- the communicative ability in the display of results
- the ability to learn as the ability to link the different dealt topics with each other and with the basic and related disciplines.
knowledge of Genetics, Genetic engineering and Biochemistry
Course contents summary
Molecular genetics techniques and their applications. Molecular analysis of genes and their products. Analysis of genomes. Molecular genetics for recombinant biotechnology.Forensic genetics.
Techniques of Molecular Genetics. Restriction endonuclease. Ligase. Vectors. Transformation and transfection. Separation and analysis of DNA molecules (density gradient centrifugation, gel electrophoresis, electron microscopy). PCR. Sequencing.
Gene cloning. Cloning strategies: function insertion, complementation of mutation, colony hybridization. Construction and analysis of libraries of DNA and cDNA. From the sequence to the gene, from gene to protein, from the protein to the gene function. The Northern blot analysis. Southern blot. Homologous genes and gene families.
From genes to genomes. The genome projects (sequencing and analysis of genomes). Functions of the genome. The reverse genetics. The gene knock out.
Molecular genetics for recombinant biotechnology. Producing organisms (bacteria, yeasts, mammalian cells). Production strategies.
The forensic genetics. DNA polymorphisms. Techniques of Analysis. Interpretation of results. Issues of law and ethics.
• Principi di Genetica, D.P. Snustad e M.J. Simmons, terza edizione, EDISES
• Genetica, B.A. Pierce, Zanichelli
• Genetica Fondamenti, P.J. Russel, EDISES
• Genetica, principi di analisi formale. Griffiths et al, settima edizione italiana, Zanichelli
• Concetti di Genetica, Klug et al, ottava edizione, Pearson Ed
• Forensic DNA typing. Biology, Technology, and Genetics of STR Markers John M Butler, Second Edition, ELSEVIER Academic Press
• La prova del DNA per la ricerca della verità. Aspetti giuridici, biologici e probabilistici Ricci Previderè Fattorini Corradi Giuffrè Editore
The course consists of lectures in which the topics of the program are presented. Each topic will be presented in experimental form, with an atempt to answer the following questions: what was the reason for the experiment, how was it carried out, what result did it led to and what is the significance of the results? The course will be supported by theoretical exercises aimed at stimulating the ability to apply the knowledge.
Assessment methods and criteria
The verification of the achievement of learning objectives takes place through questions placed in the classroom and the final exam.
The final exam, lasting 1.30 hours, is written and includes 6 open questions and exercises. The final vote derives from the sum of the scores obtained for each question (maximum 5 points per question). The score takes into account the correctness of the acquired knowledge (50%), reasoning and linking ability (30%), exhibition capacity and language properties (20%). The results of the examination are published on the Esse3 portal within a week from the examination date. Students are invited to view the exam during the student reception.