PRINCIPLES OF MICROBIOLOGICAL DIAGNOSIS
Learning outcomes of the course unit
The objectives of the course of Principles of Microbiological Diagnosis are to enable the acquisition of knowledge and understanding about the criteria for the direct and indirect laboratory diagnosis of infectious diseases caused by bacteria, fungi, protozoa and viruses and the evaluation of the in vitro susceptibility of microorganisms to antibiotics. The course also aims to provide the ability to apply in occupational contexts the knowledge and understanding acquired in the field of microbiological diagnosis.
Course contents summary
Criteria for the direct and indirect microbiological diagnosis. Microscopic examination in bacteriology, mycology, parasitology and virology. Cultural examination in bacteriology, mycology, parasitology and virology. Serological examination in bacteriology, mycology, parasitology and virology. In vitro susceptibility testing of microorganisms to antibiotics.
Criteria for the direct microbiological diagnosis. Criteria for the indirect microbiological diagnosis. Principles of direct bacteriological, mycological, parasitological and virological microscopical examination. Principles of bacteriological, mycological, parasitological and virological cultural examination. Principles of the bacteriological, mycological, parasitological and virological serological examination. Principles and methods for the execution of drug susceptibility testing of microorganisms.
M. La Placa. Principi di Microbiologia Medica. XIV ed., Società Editrice Esculapio, Bologna -EdiSES, Napoli, 2014.
The topics concerning the contents of the course will be presented and discussed through lectures with the help of iconographic material collected in PowerPoint files which, at the end of the course, will be made available to the students. During the lessons the main concepts of each topic will be exposed, emphasizing the links between the various parts of the course and encouraging the active participation of the students.
Assessment methods and criteria
The assessment of the achievement of the objectives foreseen by the course will be carried out through an oral examination. Questions relating to the contents of the course will be used to verify whether the student has achieved the objective of knowledge and understanding the scientific discipline and is able to use the technical-scientific language in an appropriate manner and to apply the knowledge acquired in occupational contexts. Failure to answer to one question, or proven inability to demonstrate at least a basic knowledge on the subject, will prevent the successful completion of the exam. Upon successful completion of the examination, a grade, expressed in thirtieths, will be attributed according to the degree of achievement of the objectives (excellent, good, fair, fully sufficient, barely sufficient), which will contribute to the final grade of the integrated course of Microbiology and Hygiene (Microbiology and Clinical Microbiology - 6 CFUs, Principles of microbiological diagnosis - 2 CFUs, General and applied hygiene - 2 CFUs), obtained on the basis of the average of the grades assigned in the different modules. The honors will be given to students who have demonstrated particular communication skills and autonomous learning. The result of the examination is communicated immediately to the student, and, after its immediate acceptance, is then published on the ESSE3 portal (https://unipr.esse3.cineca.it/Home.do).