Learning outcomes of the course unit
The Course of Biochemistry aims to enable the student to know and understand the structure, organization and function of living matter in molecular terms also in order to allow the student the ability to use the knowledge and understanding gained in identifying problems associated with the structural chemistry and structure-function relationship; metabolism and its regulation; biological information pathways.
The student at the end of the Course, using the knowledge of Organic Chemistry previously acquired, must demonstrate knowledge and understanding about:
-the interaction of drugs with biological macromolecules
-the effect of drugs on metabolism and its regulation
-the regulation of metabolism by drugs
-the disease as a result of a genetic modification of macromolecules; hypothetical therapy
In addition, students, applying the knowledge and understanding acquired, shall be able,even collaborating with other professionals, to help explain:
-the mechanism by which a drug or other health products acts in a biological process
-what are the molecular basis of diseases
-mechanism which regulate cellular homeostasis
-which food to allow normal growth, development and well-being.
The student must have sufficient technical language to explain molecular events occurring in the cell in health and /or disease
Basic knowledge of General Chemistry, Organic Chemistry,Cell Biology.
Course contents summary
First part: Protein structure and function. Proteins of special physiological interest. Enzymes. Vitamins. Lipids. Biological membranes. Nucleotides and nucleic acids. Second part: Bioenergetics, oxidative metabolism and hormonal regulation. Metabolism of carbohydrates: glycolysis, glycogen metabolism, gluconeogenesis, pentose phosphate pathway. Lipid metabolism. Amino acid metabolism. Tricarboxylic acid cycle. Oxidative phosphorylation. Metabolism of purine and pyrimidine nucleotides.Metabolism regulation.Signaling pathway. Third part: Mechanisms of DNA duplication and RNA transcription . Protein synthesis.
Introduction to the Biochemistry study
Amino acids, peptides
Three-dimensional protein structure
Myglobin and Hemoglobin
Enzymes and coenzymes
Lipids, membranes and transport
Nucleotides and nucleic acids
Bioenergetics and metabolic pathways
The citric acid cycle
Electron transport and ATP synthesis
Amino acid metabolism
Hormonal regulation and signaling pathways
DNA and RNA metabolism
D.L.Nelson,M.M.Cox:'' I principi di Biochimica di Lehninger'';J.Berg,J.L.Tymoczko,L.Stryer:''Biochimica''; M.K.Campbell, S.O.Farrell”Biochimica”; D.Voet,J.G.Voet,C.W:Pratt:''Fondamenti di Biochimica'';T.M.Devlin:''Biochimica''; R.H.Garrett,C.M.Grisham:''Principi di Biochimica''; Siliprandi,Tettamanti:''Biochimica Medica''
Oral lesson frontal carried out with the help of communication tools.
Please refer to "Reference texts" useful for the preparation of exam:
Assessment methods and criteria
The verification of the achievement of the objectives set by the Integrated Course includes a written test with open answers with time available for 2 hours and 30 minutes. Through questions regarding the course content will be determined whether the student has achieved the goal of understanding and knowledge.The answers to four questions will make it possible to ascertain whether the student has achieved the goal of appying the acquired knowledge. The first question will concern the structural chemistry and the structure-function relationship, the second the metabolism and the biological information pathways , the third regulation and/or deeping of metabolic pathways, the fourth topic of Applied Biochemistry.Each answer will be evaluated with a score from 0 to 30/30 praise.The final evaluation will consist of adding the vote obtained in the answer to each individual question and dividing by the number of questions. To pass the exam it is necessary to have a grade higher than eighteen in the Applied Biochemistry response.