Research and identification of viral agents using traditional techniques
LEARNING OUTCOMES OF THE COURSE UNIT
The aim of the course is to deepen the student's knowledge about specialized topics of Microbiology and to provide the ability to use this knowledge in the workplace.
Passing exams of previous years in the number established for the bachelor course in TLB is needed to access this course.
COURSE CONTENTS SUMMARY
Traditional techniques for identification of viral agents causing diseases in humans.
It is a theoretical-practical course, with lessons carried out mainly in laboratory. Supplementary didactic materials will be provided by the course teachers.
ASSESSMENT METHODS AND CRITERIA
The outcomes of learning will be verified through a written examination. By questions regarding the topics of the course it will be determined whether the student has achieved the goal of knowledge and capability to apply it in the workplace.
The topics of the course will be presented and discussed through theoretical and practical lessons.
"Identification of viral agents using traditional techniques."
In vitro cultivation of viruses: diploid cell cultures and cell lines, preparation of cellular substrates. Virus-induced cytopathic effect: definition, causes, examples, virus identification.
Immunofluorescence reaction: direct and indirect immunofluorescence, research and identification of viral proteins.
Transmission electron microscopy: general principles, grids preparation, negative staining, virus observation.