RECOMBINANT PROTEINS
Learning outcomes of the course unit
The course aims at providing students with the fundamentals to understand issues related to the production of recombinant proteins. Through practicals students will become familiar with the most important techniques for the production of recombinant proteins.
Prerequisites
Basic knowledge of biochemistry and biology.
Course contents summary
Production of recombinant proteins for basic research and for therapeutic applications.
Overview of different expression systems.
Methods for the production of recombinant proteins.
Course contents
Recombinant protein production: the choice of the optimal expression system.
Overview of therapeutical recombinant proteins.
Recombinant proteins as drugs: case studies.
Methods for the production of recombinant proteins in E. coli:
- gene identification and optimization of the coding sequence
- PCR
- cloning and analysis of recombinant clones
- cultivation of bacteria
- inducible expression in bacteria
- expression analysis and improvement
- protein extraction and purification
Recommended readings
Selected scientific papers and a copy of the slides can be downloaded from the Elly website.
Metodologie Biochimiche - Bonaccorsi di Patti, Contestabile e Di Salvo. Casa Editrice Ambrosiana, 1a edizione.
Biotecnologie Molecolari- Terry A. Brown. Zanichelli, 1a edizione.
Teaching methods
Lectures will deal with the principles of recombinant protein production and their application in different fields, especially the pharmaceutical field. Practicals will stimulate the active acquisition of the basic knowledge on recombinant protein production.
Assessment methods and criteria
Oral examination
