APPLIED BIOCHEMISTRY

General principles of biochemical experimental investigation
Biological materials
Methods of separation and purification of proteins and other molecules of biochemical interest: centrifugation, salt fractionation, dialysis and ultrafiltration.
Chromatographic techniques. Principles and modes of chromatography. Chromatografic performance parameters. Adsorption chromatography; ion-exchange chromatography;molecular exclusion (gel filtration) chromatography; affinity chromatography; high performance liquid chromatography (HPLC);gas-liquid chromatography.
Electrophoretic techniques. General principles. Support media and detection methods. Electrophoresis of proteins and of nucleic acids. Isoelectric focusing. Capillary electrophoresis.
Spectroscopic techniques. Properties of electromagnetic radiation and interaction with matter. Ultraviolet and visible light spectroscopy. Spectrofluorimetry. Applications in biochemical analysis.
Enzyme assay and kinetic analysis.
Immunochemical techniques. Immunoglobulin structure. Production and purification of polyclonal and monoclonal antibodies. Immunoprecipitation . Labeling antibodies. Enzyme-
immunoassay.Immunoblotting.
Radioisotope techniques and radioimmunoassay (RIA).
Potenziometric and polarographic techniques