Learning outcomes of the course unit
The student is asked to understand and learn the content of the course. Attendance to lessons, which is mandatory, will allow the student to acquire skills appropriate to describe, communicate effectively, use independence of judgment and comment features, functions, structure and the metabolic role of the main bio-molecules (proteins, nucleic acids, enzymes and co-enzymes, water and fat soluble vitamins); the mechanisms of metabolic energy production, energetic and structural homeostasis of living cells; structure and function of eukaryotic genes, replication of DNA, gene expression, DNA repair; the main metabolic pathways and their regulation; structure and functions of hormones, endocrine regulation of the metabolism; metabolic and biochemical correlations between organs and tissues under normal and pathological conditions; the molecular basis of human diseases; research approaches to molecular medicine and translational research.
Molecular basis of genetic information. Composition and structure of nucleic acids. Chromatine, chromosomes and genes. DNA replication, DNA recombination, DNA damage and mutation, DNA repair. Cell aging and cancer. Types of RNA: structure and functions. Genetic code and its properties. Mechanisms and regulation of transcription. Protein synthesis and its regulation. Regulation of gene expression and fate of post-synthetic proteins. Molecular biology techniques: DNA sequencing and cloning, restriction enzymes and restriction maps, transgenes and transgenic organisms. Recombinant DNA technology in forensic medicine. Gene therapy.
Nelson DL, Cox MM: I principi di Biochimica di Lehninger, Zanichelli, Bologna.
JD Watson et al.: Biologia Molecolare del gene. Zanichelli, Bologna.
Devlin TM: Biochimica con aspetti clinici. Wiley-Liss, New York
The course will be held with oral lectures and seminars to deep single issues, and will make use of multimedia systems. During the lessons, students will have the opportunity to discuss the key aspects of the course. Teachers will be available throughout the duration of the course to answer questions and support students during their training with individual meetings by appointment.