Techniques for the laboratory diagnosis of infections caused by viral agents infecting the liver
Learning outcomes of the course unit
The course aims to enable the student to know and understand the principles and practice of the main methods for the laboratory diagnosis of the infections due to hepatitis A, B, C, D and E viruses and provide the ability to apply in occupational contexts the knowledge and understanding acquired in the specific field.
Prerequisites
Passing examinations of Microbiology I and Clinical Microbiology II year of
degree
Course contents summary
Principles and practice of the laboratory investigations for the detection/quantification of the conventional markers of infections with hepatitis A, B, C, D and E viruses and the respective viral nucleic acids. Principles and practice of the reverse hybridization assay after amplification for genotyping hepatitis B and C viruses and for identifying drug-resistant hepatitis B virus.
Meaning and use of quality controls for the assays above mentioned.
Recommended readings
Visual aids made available by the teacher;
MICROBIOLOGIA MEDICA – Gli agenti infettivi dell’uomo: biologia, azione patogena, laboratorio.
VIROLOGIA. M. Bendinelli, C. Chezzi, G. Dettori, N. Manca, G. Morace, L. Polonelli, M.A. Tufano. MONDUZZI EDITORIALE. Anno 2007.
Teaching methods
Matters relating to the course content will be illustrated and discussed through interactive lessons.
Assessment methods and criteria
Certificate of attendance