CLINICAL BIOCHEMISTRY CLINICAL MOLECULAR BIOLOGY
Learning outcomes of the course unit
The aim of this corse is to provide a clear-cut on the generation of laboratory data so as it is clear to the clinician its reliability, the crucial steps source of possible errors, the value of a lab test regarding the planned clinical path, the consistency and plausibility of those many lab data obtainable (from the more recent lab instrumentations) according to the clinician request (for example as function of the diagnostic hypothesis).
Besides the obligatory prerequisites, it is appropriate that the student is able to manage the basic concepts of Biochemistry, Physiology and General Pathology.
Course contents summary
Pre-analytical, analytical and post-analytical variability. Types of biological samples and optimal procedures to collect them. Variables affecting laboratory data
The concept of normal values in biology and the risk functions (normal values stated by statistical calculations or by biological observations; desirable values and risk function; decisional values for diagnostic or therapeutic purpose).
Interpretation of the complete urinalysis test.
Interpretation of enzymatic and iso-enzymatic tests like auxiliary diagnostic tool in some specific organ disease.
Laboratory test modifications by diseases affecting purine metabolism.
Markers of function or damage of some organs and systems. Index of cytolysis. Laboratory test modifications by diseases affecting the hydro-electrolyte balance. Interpreting the variations of acid-base parameters and possible pathological conditions.
Interpretation of the modifications of plasma protein profile.
- Kellerman G. Valori anormali di laboratorio. Mc Graw Hill
- Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Elsevier Saunders
Assessment methods and criteria