HUMAN AND MODEL ORGANISMS MOLECULAR GENETICS GENOTOXICITY
Learning outcomes of the course unit
Aims of the course is the study of genetic approaches applied to genomics and functional analysis in eukaryotes model organisms. Particular attentions will be paied to model organisms in fundamental and biotechnologic applications.
The course will study molecular aspect of human genetics and application to toxycology
Course contents summary
YEAST AS MODEL ORGANISM
Forward e reverse genetics- Genome sequencing in S. cerevisiae- Comparative genomics in yeasts- S. cerevisiae deletant collection and use of null mutants in functional analysis- Methods for gene interaction studies applied to human diseases - Yest as model organism for the study of mitochondrial genetics- Yeast as model organism for the study of mitochondrial diseases.
FUNCTIONAL ANALYSIS IN PLANTS
Caenorhabditis elegans AS MODEL ORGANISM
Forward e reverse genetics in C. elegans. Functional analysis by RNAi - C. elegans as model organism for the study of human pathologies.
Drosophila melanogaster AS MODEL ORGANISM
Forward e reverse genetics in D. melanogaster. - D. melanogaster as model organism for the study of human pathologies. HUMAN GENETICS. HUMAN GENETIC DISEASES.
PHARMACOGENETICS. GENE THERAPY.GENOTOXICOLOGY
MODEL ORGANISMS GENETICS:
Philip Meneely "Analisi Genetica Avanzata" ED. McGraw-Hill
ORIGINAL PAPERS INDICATED BY THE TEACHER
MR CUMMINGS "Eredità Principi e problematiche della genetica umana" EdiSES 2009
T.STRACHAN, A.P.READ "Genetica Molecolare Umana" – Zanichelli 2012. L.MIGLIORE – Mutagenesi Ambientale – Zanichelli 2004
frontal lectures and exercises
Assessment methods and criteria
Tests during the course and final oral examination