DESIGN OF BIOTECHNOLOGICAL VACCINES
Learning outcomes of the course unit
Acquisition of essential knowledge about currently available biotechnogical vaccines and experimental biotechnological approaches for the rationale design of new vaccines against infectious diseases.
Acquisition of the current viral gene manipulation techniques relative to gene therapy.
Students are required to have a knowledge of basic molecular biology.
Course contents summary
Currently available biotechnogical vaccines and vaccine technologies. Experimental biotechnological approaches for the rationale design of new vaccines against infectious diseases.
Viral Vectors in gene therapy and immunotherapy. Retrovirus:
a) Molecular aspects of replication
b) Vector structure: oncoretrovirus (MMLV) and lentivirus (HIV)
c) Packaging strategies
d) Targeting and pseudo-typification strategies and mechanisms (VSV-G)
e) Cell transduction and expression control.
Scientific reviews on specific topics. Lecture notes.