BIOMATERIALS AND BIOCOMPATIBLE SYNTHETIC MATERIALS
|OSTEOGENESIS AND CONDROGENESIS: MODELS OF BIOCOMPATILITY IN VIVO AND IN (3 credits)||OSTEOGENESIS AND CONDROGENESIS: MODELS OF BIOCOMPATILITY IN VIVO AND IN (2 credits)|
|BIOMATERIALS AND BIOPROSTHESIS unit A (1 credits)||MEDICINAL PRODUCTS FOR ADVANCED THERAPY (2 credits)|
|BIOMATERIALS AND BIOPROSTHESIS UNIT B (2 credits)|
Learning outcomes of the course unit
The aim of the course is give specific knowledge and an up-to-date account of current knowledge in biology and pathophysiology of articular cartilage and bone and in biotechnological techniques, focusing on tissue repairing. With a practical approach the students will acquire a core professional training in primary cell culture methods and techniques and also they will acquire capacity to apply systems to culture articular chondrocytes and also protocols to study osteogenesis in vivo. Students will also acquire knowledge on natural and synthetic polymers and scaffols for biomedical use, expecially for bone reparing, vascular surgery, surgical hemostasis and ocular implants.
Course contents summary
1) Osteochondrogenesis: in vivo and in vitro models of biocompatibility(5 CFU)
Lecturer: Prof. Paolo Borghetti (VET/03)
(CFU: 1 CFU lesson (8 hours) + 1 CFU praticals (8 hours)
Anatomy , histology and biomechanics of articular joints. Structure, biology and metabolism of articular cartilage.
Physiopathology of tissue cartilage metabolism. The molecular basis of chondrocyte differentiaiton and chondrogenesis. Biomaterials for cartilage reparing
Isolation of chondrocytes from articular cartilage tissue.
Adhesion and tridimensional models of primary chondrocyte culture.
Tecnique for maintening differentiation of articular chondrocytes in culture.
Dott. Cacchioli Antonio (VET/01) (3 CFU),
Theoretical part (1,5 CFU- 12 hours)
Locomotor system: classification of bones and principles of biomechanic
- Forms of bony tissue
- Morphological and structural characteristics
- Deposition and remodeling dinamics
Definition and classification of biomaterials employed in orthopaedic and dental field
Experimental protocols for evaluation of osteointegration
Notions of legislation on animal experimentation
Methods for the osteintegration evaluation: bone vital markers, polarized light microscopy analysis, histochemical and immunohistochemical reactions, histological stains.
The practical activity (1, 5 PrCFU, 12 hours) will focused on:
- Histological techniques for processing undecalcified bone tissue with implants
- Histolgical stains, Histochemical and Himmunohistochemical reactions
- Observation of histological sections by polarizing light and fluorescence optical microscope
- Digitalization and image analysis.
Lecturer: Prof. Filippo Maria Martini (VET/09: 2 CFU)
• Experimental protocols for the in vivo biointegration evaluation of materials to be used in the orthopedic and maxillo-facial field.
• Concepts of animal experimentation legislation.
• Choice criteria of experimental animal.
• Definition and classification of biomaterials: metals and metal alloys, ceramics, thermoplastic and thermohardening polymers, composites, biodegradable and bioactive materials.
• Materials in the orthopedic and maxillo-facial field (steel, titan, PDLLA, covering materials, cements).
• Clinical use of biomaterials. Orthopedics: articular prosthesis, bone cement, ligament substitution, internal and external fracture fixation.
• Biomaterial functionality monitoring after implants. Periprosthetic infection. Orthopedic prosthesis mobilization.
• European legislation on medical devices.
2) Biomaterials and bioprotesis (1 CFU)
a) Dott. Luigi Bonati (MED/18 0,5 CFU: 4 ore)
Biomaterials in general surgery, vascular surgery and surgical hemostasis
Prostheses biocompatible in addominal wall surgical repair, in incisional hernias, and in addominal hernias repair.
Vasculars prostheses and patches in vascular surgery.
Hamostatic medications in abdominal, vascular, and thoracic surgery.
b) Dott. Claudio Macaluso (0,5 CFU: 4 ore)
• Intraocular lenses (IOLs): optical, biological and surgical requirements in designing substitutes of the natural crystalline lens.
• Corneal implants and artificial cornea.
• Setons and other implants for the therapy of glaucoma.
• Sub- and pre-retinal prosthesis: optoelectronics to restore visual transduction and reverse blindness.
1) Scientific reviews on course topics
2) Lab experimental protocols