Students will be able to develop and optimize the most proper analytical method, evaluating its quality parameters.
Knowledge of basic principles of general and organic chemistry.
Course contents summary
The course will provide student with knowledge of theoretical principles and applications of instrumental analysis techniques (spectrophotometric, chromatographic and mass spectrometric methods).
Introduction to analytical chemistry: qualitative and quantitative analysis.
Validation of analytical methods. Quality parameters of analytical methods: precision, accuracy, limit of detection, limit of quantification, linearity, selectivity, sensibility.
Spectrophotometric methods. rotational, vibrational and electronic transitions. Absorption, emission, fluorescence. Lambert-Beer's law.
Instruments for spectrophotometric analysis: sources, monochromators and detectors
UV-Vis spectroscopy: principles, instrumentation and applications in qualitative and quantitative analysis
IR spectroscopy: principles, instrumentation and applications in pharmaceutical analysis
Chromatographic methods. General principles and theory of chromatographic separation. Fundamental parameters in chromatography.
Gas-chromatography (GC): theoretical principles. Instrumentation. Qualitative and quantitative analysis.
High pressure liquid chromatography (HPLC): adsorption chromatography, partition chromatography, size-exclusion chromatography, ion-exchange chromatography. Instrumentation.
Qualitative and quantitative analysis.
Mass spectrometry: theoretical principles. Instrumentation: ion sources, mass analyzers, detector. Qualitative and quantitative analysis. GC-MS and HPLC-MS. Tandem mass-spectrometry.