Learning outcomes of the course unit
The course of Applied Biochemistry is a part of teaching or course-integrated Biochemistry-Applied Biochemistry.
The Course aims to enable the student to know and understand the general principles of experimental biochemistry, biological materials, principles and equipment used for analytical and preparative purposes including for the purpose of allowing the student the ability to use the knowledge and understanding gained to understand the significance of biochemical investigation, discipline-based research, and appropriated the principles and methods normally used in biochemical laboratory.
The student at the end of the Course, using the knowledge of Organic Chemistry previously acquired, must demonstrate knowledge and understanding about:
-material used in biochemical experiments
-preparative and analytical separation techniques
-techniques based on biospecific interactions
In addition, students, applying the knowledge and understanding acquired, shall be able,even collaborating with other professionals, to help explain:
-how to investigate the mechanism of action of a drug
-investigate as to understanding the molecular basis of diseases
-how to study the properties of an enzyme marker of a disease
-how to measure an antigen in a biological fluid complex
Before taking the Applied Biochemistry exam, the Organic Chemistry exam must have been passed.
Course contents summary
First part:general criteria in biochemical experimentation. Biological materials.
Second part:methods of separation and purification of proteins and other molecules of biochemical interest: centrifugation, salting out, dialysis, chromatography,electrophoresis.
Third part: biochemical applications of visible and ultraviolet spectroscopy. Study of enzymatic activity. Immunochemical, radioisotope and radioimmunologic techniques. Potentiometric and polarographic techniques Overview of molecular biology.
K.Wilson,J.Walker:''Biochimica e Biologia molecolare. Principi e tecniche'';
R.Reed,D.Holmes,J.Weyers:''Metodologie di base per le Scienze biomolecolari'';C.De Marco,C.Cini:”Principi di metodologia biochimica”.
Oral lesson frontal carried out with the help of communication tools.
Assessment methods and criteria
Verify the achievement of the objectives referred Course requires a written examination. Through questions regarding the course content will be determined whether the student has achieved the goal of knowledge and understanding the content. By questions about the general principles of biochemical investigations and biological materials used, methods of separation and purification of proteins and other molecules of biochemical interest, the principles and application of spectroscopic techniques and immunochemical techniques will be determined whether the student has attained the aim of applying the acquired knowledge .