HISTOLOGY AND EMBRYOLOGY GENERAL VETERINARY
Learning outcomes of the course unit
The course of "General Veterinary Histology and Embryology" is a part of the integrated course of "Histology and Embryology Zoology." The course aims to enable the student to know and understand the concepts of cytology, histology and embryology and to acquire the knowledge for the recognition of the various tissues by light microscopy. In addition to understanding the dynamics of development from gametogenesis to the formation of the embryonic three germ layers, changes in the birth of the fetal circulation, the dynamics of the formation of the fetal membranes and placentation in animals of veterinary interest. At the end of the course, the student will have to demonstrate to know and understand the notions teached on the various topics during the frontal teaching and practical activity. Particularly, the student have to: correlate the structural features to the functions both at the subcellular, cellular and tissue levels; apply this knowledge in the recognition of the various tissues through optical microscope analysis; know the gametogenesis, the formation of the three germ layers, the fetal circulation, the fetal membranes and placentation in animals of veterinary interest.
Specific knowledge required for the entrance examination or alternatively recovery of the debt of Biology.
Course contents summary
The course includes theoretical lessons that take place in the classroom and practical lessons that take place in the laboratory of histology and in the Room of Microscopic Anatomy .
The course consists of 3 parts. In the first part, that is devoted to cytology, the structure of the eukaryotic cell is recalled and in-depth analized. In the second part, that is devoted to general embryology, are given knowledge on embryological development from gametogenesis to the formation of the three germ layers.
In addition, teaching on the fetal membranes, the placentation and the fetal circulation and the related changes at birth for the species of veterinary interest is done. In the third part, that is devoted to histology, the teacher provides knowledge on structural and functional characteristics of the various tissues and on the techniques and equipment necessary for the preparation of histological slides and their analysis.
In the practical lessons the student is exercised in recognition of tissues by light microscopy. For the practical activity is required the distribution of students in different groups, the number of groups is related to the total number of students and on the lab and room capacity.
The Biological membrane and the Plasma membrane: structure.
Cytoplasmic organelles: Smooth and Rough Endoplasmatic Reticulum, Ribosomes, Golgi Apparatus, Lysosomes, Peroxisomes, Mitocondria, Cytoskeleton: Microtubules, Microfilaments, Intermediate filaments; Centrioles, Centrosome.
Interphase Nucleus: Nuclear Envelope, Nucleoplasm: Nuclear Matrix, Chromatin: Euchromatin, Heterochromatin and Nucleolus.
Intercellular and cell to matrix junctions: Occluding junctions; Adherens Junctions: Adhesion belt, Desmosomes, Hemidesmosomes and Focal adhesions; Communicating junctions. Junctional complex.
Specializations of the apical edge of plasma membrane; structural features and localizations: Microvilli, Cilia, Sterociglia.
Stages of cell cycle. Proliferation state, potency and cell differentation.
Remind of cell division: Mitosis and Meiosis. Definition of programmed cell death.
Spermatogenesis e Oogenesis.
From spermiogenesis to anfimixis.
Gastrulation: formation of the three germ layers.
Derivates of the embryonic germ layers.
Placenta and extraembryonic membranes in mammals of veterinary interest.
Fetal circulation and adaptations to postnatal life.
Embrionyc anexa in birds.
Instruments and methods for cell and tissue observation and study.
Methods for the preparation of a histology section: sample harvest, fixation, dehydration, embedding, sectioning, staining and mounting.
Elements of histological staining.
Structural organization of organs: Pipe organ, Parenchymatous organs, Laminar organs.
Epithelium Tissue: Embryonic origin and distinctive tissue characteristics.
Lining Epithelium: Classification and localizations: Simple epithelium, Pseudostratified Epithelium, Stratified Epithelium, Sensory Epithelium, Specialized Epithelium.
Glandular Epithelium Exocrine glands: Localizations and classification relative to: number of secretory cells, disposition respect the organ wall, secretory units characteristics, ducts system characteristics, method of secretion, secetory product.
Endocrine glands: Localizations and classification relative to: number of secretory cells, structural features: Solid cell cord gland, Follicular gland, diffuse cells gland.
Connective Tissue: Embryonic origin, Cells and extracellular matrix, Localizations and classification.
Embryonic Connective Tissue.
Adult Connective Tissue:
Proper Connective Tissue:
Collagen Connective Tissue:
Regular: right-angle fibres, parallel fibres.
Reticular Connective Tissue.
Elastic Connective Tissue.
Pigmented Connective Tissue.
Adipose Connective Tissue: Brown, White.
Specialized Connective Tissue:
Classification and localizations: Hyaline Cartilage, Fibrocartilage, Elastic Cartilage.
Features of cartilage cells and matrix.
Classification and localizations: Woven bone, Lamellar bone, Compact bone, Spongy bone.
Features of bone cells and matrix; Compact and Cancellous or Spongy bone: Bone lamellae, Osteons.
Bone Tissue and Bones:
Periosteum, Endosteum,. Medullary cavità.
Bone marrow: Red, Yellow, Gelatinous.
Diaphysis > Body. Epiphyses > Extremities. Metaphyses
Fluid Ground Substance Connective Tissue. Blood and Lymph:
Cell and plasma components.
Structural characteristics of:
Leucocytes: Monoytes, T Lymphocytes, B Lymphocytes,
Granulocytes: Neutrophils, Eosinophils, Basophils,
Muscle Tissue: Embryonic origin and classification of muscle tissue:
Smooth Muscle Tissue,
Striated Muscle Tissue,
Striated Cardiac Muscle Tissue.
Nervous Tissue. General details on nervous tissue and nervous system.
Histogenesis of nervous tissue.
Myelin and nonmyelinated nerve fibres; Nerve fibers.
Visit to the histological laboratory.
Technical procedure for paraffin embedded histology.
Methods to use the components of optic microscope.
Resolution, magnification, penetration depth.
Focus and observation of histologic specimen.
Identification of histologic staining.
Identification of nucleus and cytoplasm.
Identification of the Lining Epithelium tissue:
Simple squamous Epithelium.
Simple cuboidal Epithelium.
Simple columnar Epithelium.
Pseudostratified cuboidal or columnar, ciliated Epithelium.
Stratified squamous Epithelium
Stratified squamous, more or less keratinized Epithelium.
Stratified squamous, differential keratinized Epithelium.
Stratified squamous, keratinized Epithelium: Skin.
Identification of the Glandular Epithelium tissue:
Acinar–branched gland (Sebaceous gland).
Coiled-tubular gland (Sudoriparous gland).
Compound tubulo-acinar, serous secreted gland.
Compound tubulo-acinar, mixed secreted gland.
Simple tubular gland.
Branched tubular gland.
Simple unicellular gland.
Labirinthic gland: liver.
Solid cell cord gland.
Identification of the Connective tissue:
Collagen Loose Connective.
Collagen Dense Irregular Connective.
Collagen Dense Regular Connective.
Spongy and Compact Bone.
Blood (Peripheral blood smear)
Identification of the Muscle tissue:
Smooth Muscle Tissue.
Striated Muscle Tissue.
Striated Cardiac Muscle Tissue.
Identification of the neuron: cell body and nerve cell processes.
In addition to the reference texts listed below, are available to students, in the special sites dedicated to them within the university homepage, accessible through identification and password, pictures of the histological slides used during the theoretical and practical activities:
PELAGALLI G.V., e coll.: Embriologia, Morfogenesi e Anomalie dello Sviluppo, Idelson - Gnocchi, Napoli, 2009.
BARBIERI M., CARINCI P.: Embriologia, Ambrosiana, Milano.
BARONE R.: Anatomia Comparata dei Mammiferi Domestici, Vol 4, Edagricole, Bologna.
DE FELICI M., e coll.: Embriologia Umana - Morfogenesi – Processi molecolari - Aspetti Clinici, Piccin, Padova, 2009.
LATSHAW W. K.: Lo sviluppo degli Animali Domestici – Embriologia ad Orientamento Clinico, Ambrosiana, Milano.
NODEN DREW M., DE LAHUNTA A.: Embriologia degli Animali Domestici – Sviluppo normale e Malformazioni congenite, Edi-ermes, Milano.
-Cytology and Histology:
ADAMO S., CARINCI P., MOLINARO., SIRACUSA G., STEFANINI M., ZIPARO
E.: Istologia di V. MONESI, Piccin, Padova.
CASASCO E.: Citologia Istologia, La Goliardica Pavese, Pavia
COOPER G. M., HAUSMAN R. E.: La cellula un approccio molecolare, Piccin, Padova
ROSS M. H., PAWLINA W., BARNASH T. A.: Atlante di Istologia e Anatomia
Microscopica, Ambrosiana, Milano, 2010.
BACHA W. J., WOOD L. M.: Atlante a colori di istologia veterinaria, Delfino, Roma.
YOUNG B., LOVE J.S., STEVENS A., HEATH J. W.: Wheater – Istologia e anatomia microscopica, Elsevier Masson, Milano.
The course includes theoretical lessons that take place in the classroom and practical lessons that take place in the laboratory of histology and in the Room of Microscopic Anatomy, where the student is exercised in recognition of tissues by light microscopy. For the practical activity is required the distribution of students in different groups, the number of groups is related to the total number of students, on the lab and room capacity and on the possibility of allowing each student to operate independently. During the academic year 2012-13 the students were divided in 2 groups each of which has carried out 20 hours of practical lessons.
Assessment methods and criteria
The final exam, structured to evaluate the achievement of the objectives of the course, include a practical and an oral tests.
The practical test consist on the recognition of tissues within an histological slide by the optical microscope and produce a written scheme with the description of the visualised tissue
The oral examination is based on the discussion of the written scheme from the practical test and on the answers to 3 questions, one for each part of the course, randomly identified by the computer.